Steinholt H C, Chandler J E, Baron R A, Adkinson R W
Department of Dairy Science, Louisiana State University Agricultural Center, Baton Rouge 70803.
J Dairy Sci. 1994 May;77(5):1239-50. doi: 10.3168/jds.S0022-0302(94)77063-6.
The objective was to evaluate bull differences in chromosomal and spermatozoal areas related to the occurrence of the bovine leukocyte adhesion deficiency syndrome. Lymphocyte chromosomes from 30 Holstein bulls and 2 Holstein heifers were measured using image analysis and computer-enhanced video-microscopy. Spermatozoal head areas from 29 of the 30 bulls were measured. Autosomal rank was based on decreasing area. Average total autosomal areas were not the same across bulls. One group of bulls had significantly smaller average chromosomal areas than the others; this group carried bovine leukocyte adhesion deficiency syndrome. Area measures of spermatozoal heads showed that bulls with bovine leukocyte adhesion deficiency syndrome had significantly larger head areas than normal bulls. Lymphocyte chromosomes from 3 cattle that were homozygous for bovine leukocyte adhesion deficiency syndrome were significantly smaller than chromosomes from syndrome heterozygotes. Carrier identification was improved by the use of autosomal and sperm area measurements in addition to pedigree evaluation.
目的是评估与牛白细胞黏附缺陷综合征发生相关的染色体和精子区域的公牛差异。使用图像分析和计算机增强视频显微镜测量了30头荷斯坦公牛和2头荷斯坦母牛的淋巴细胞染色体。对30头公牛中的29头测量了精子头部区域。常染色体排序基于面积递减。不同公牛的平均常染色体总面积不同。一组公牛的平均染色体面积明显小于其他组；该组携带牛白细胞黏附缺陷综合征。精子头部的面积测量表明，患有牛白细胞黏附缺陷综合征的公牛的头部面积明显大于正常公牛。3头牛白细胞黏附缺陷综合征纯合子牛的淋巴细胞染色体明显小于综合征杂合子的染色体。除系谱评估外，使用常染色体和精子面积测量可改善携带者的鉴定。
