Macpherson L M, MacFarlane T W, Aitchison T C, Stephen K W
Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, Scotland, United Kingdom.
J Dent Res. 1990 Nov;69(11):1706-11. doi: 10.1177/00220345900690110101.
This study describes the predominant cultivable microflora of three-week-old plaque samples obtained from human enamel sites, on the basis of microbial identification of over 9000 fresh isolates. Lower removable appliances, on which were mounted enamel sections and slabs, were worn by five young adult subjects under three experimental protocols. These were (1) 'normal' plaque conditions, (2) extra-oral sucrose applications nine times daily, and (3) inoculation of each subject's own mutans streptococci onto the enamel test sites and sucrose applications, as described above. With the exception of slightly higher proportions of Gram-negative bacilli associated with slab plaque following sucrose application, no significant differences in percentage or absolute counts of organisms were found between normal and sucrose plaques. The inoculation of mutans streptococci, combined with extra-oral sucrose applications, was associated with significantly higher percentages and absolute mean counts of both mutans streptococci and lactobacilli, and lower proportions of S. sanguis and S. oralis. Although the isolation frequency of mutans streptococci increased in all subjects and the overall mean proportion rose following inoculation, considerable inter-subject variation was seen in mean percentage counts of these organisms isolated from the three-week plaque samples.
本研究基于对9000多个新鲜分离株的微生物鉴定，描述了从人类牙釉质部位获取的三周龄菌斑样本中主要的可培养微生物群落。五名年轻成年受试者按照三种实验方案佩戴安装有牙釉质切片和平板的下颌可摘矫治器。这三种方案分别是：(1)“正常”菌斑环境；(2)每天九次口腔外涂抹蔗糖；(3)将每个受试者自身的变形链球菌接种到牙釉质测试部位并涂抹蔗糖，如上所述。除了涂抹蔗糖后平板菌斑中革兰氏阴性杆菌的比例略高外，正常菌斑和蔗糖菌斑在微生物百分比或绝对计数上没有发现显著差异。接种变形链球菌并结合口腔外涂抹蔗糖，与变形链球菌和乳酸菌的百分比及绝对平均计数显著升高以及血链球菌和口腔链球菌比例降低有关。尽管所有受试者中变形链球菌的分离频率均增加，接种后总体平均比例上升，但从三周龄菌斑样本中分离出的这些微生物的平均百分比计数存在相当大的个体间差异。
