Nyvad B, Kilian M
Department of Oral Anatomy, Royal Dental College, Aarhus, Denmark.
Caries Res. 1990;24(4):267-72. doi: 10.1159/000261281.
This study compared the initial (4 h) microflora on enamel in 7 caries-active and in 7 caries-inactive adolescents. In both groups the microflora was dominated by streptococci which comprised 61 and 78% (median values) of the total viable counts in caries-active and caries-inactive individuals, respectively (p less than 0.01). Identification of a total of 700 streptococcal isolates according to a recently revised classification showed that the predominant streptococci belonged to the species Streptococcus oralis, Streptococcus mitis biovar 1, and Streptococcus sanguis. Early plaque from caries-inactive individuals differed from that of caries-active individuals by significantly higher proportions of S. sanguis (p less than 0.05) and IgA1 protease producing streptococci (p less than 0.05). In caries-active individuals, there was a tendency to elevated levels of S. mitis biovar 1 (p less than 0.10). In addition, caries-active individuals were colonized by significantly higher numbers of mutans streptococci on the enamel surfaces (p less than 0.01). However, in both groups Streptococcus mutans (serotype c) comprised less than or equal to 2% of the early streptococcal flora. Streptococcus gordonii, S. mitis biovar 2, and Streptococcus salivarius were present in low proportions and did not show differences in distribution that could be related to caries activity. The observed differences in the composition of the early streptococcal microflora may be a factor that governs the eventual cariogenic potential of dental plaque.
本研究比较了7名患龋活跃和7名患龋非活跃青少年牙釉质表面的初始（4小时）微生物群落。两组中的微生物群落均以链球菌为主，在患龋活跃和患龋非活跃个体中，链球菌分别占总活菌数的61%和78%（中位数）（p<0.01）。根据最近修订的分类标准对总共700株链球菌分离株进行鉴定，结果显示，主要的链球菌属于口腔链球菌、缓症链球菌生物1型和血链球菌。患龋非活跃个体的早期牙菌斑与患龋活跃个体的早期牙菌斑不同，前者血链球菌（p<0.05）和产生IgA1蛋白酶的链球菌比例显著更高（p<0.05）。在患龋活跃个体中，缓症链球菌生物1型水平有升高趋势（p<0.10）。此外，患龋活跃个体牙釉质表面变形链球菌的定植数量显著更多（p<0.01）。然而，在两组中，变形链球菌（血清型c）占早期链球菌菌群的比例均小于或等于2%。戈登链球菌、缓症链球菌生物2型和唾液链球菌所占比例较低，且在分布上未显示出与患龋活性相关的差异。早期链球菌微生物群落组成的观察差异可能是决定牙菌斑最终致龋潜力的一个因素。
