Wang Zhi-Gang, Wang Xi-Jun, Sun Hui, Chen Li, Ma Chao-Mei
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China.
J Asian Nat Prod Res. 2012;14(2):176-81. doi: 10.1080/10286020.2011.631132.
In a series of studies on the metabolism of iridoid compounds, we investigated the metabolic fate of swertiamarin (1) in Wistar rats. Liquid chromatography/ion trap mass spectrometry detected new nitrogen-containing metabolite gentiandiol (3) in rat plasma. The structure of the metabolite was unequivocally identified by comparing the retention time as well as the mass spectrum with those of authentic compound, which was synthesized from swertiamarin (1). The transformation of swertiamarin to nitrogen-containing metabolite gentiandiol (3) in vivo was verified for the first time. Understanding of this unique metabolic pathway may shed light on clinical efficacy of swertiamarin (1) and will also assist in studies for the metabolism of other natural iridoids in vivo.
在一系列关于环烯醚萜类化合物代谢的研究中，我们研究了獐牙菜苦苷（1）在Wistar大鼠体内的代谢命运。液相色谱/离子阱质谱法在大鼠血浆中检测到了新的含氮代谢产物龙胆二醇（3）。通过将保留时间以及质谱与由獐牙菜苦苷（1）合成的 authentic 化合物的保留时间和质谱进行比较，明确鉴定了该代谢产物的结构。首次证实了獐牙菜苦苷在体内向含氮代谢产物龙胆二醇（3）的转化。对这一独特代谢途径的了解可能会揭示獐牙菜苦苷（1）的临床疗效，也将有助于体内其他天然环烯醚萜类化合物代谢的研究。
