Stefanska J, Sarniak A, Wlodarczyk A, Sokolowska M, Pniewska E, Doniec Z, Nowak D, Pawliczak R
Department of Immunopathology, Medical University of Lodz, Lodz, Poland.
Exp Lung Res. 2012 Mar;38(2):90-9. doi: 10.3109/01902148.2011.649823.
Asthma is an inflammatory airway disease, and oxidative stress was proven to be involved in its pathogenesis. Apocynin effectively inhibits the main source of reactive oxygen species (ROS)-nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-by blocking its activation. The aim of this study was to investigate the effect of inhaled apocynin on ROS and RNS (reactive nitrogen species) concentration in 14 nonsmoking mild asthmatics. Effects of nebulized apocynin (0.5 mg/mL) were assessed in exhaled breath condensate (EBC) after 30, 60, and 120 minutes, and safety parameters have been analyzed. Apocynin significantly decreased H2O2 concentration in EBC in comparison with placebo after 60 and 120 minutes. Moreover, apocynin significantly reduced NO(-2) concentration 30 and 60 minutes after nebulization and caused a significant decrease of NO(-3) concentration in EBC 60 and 120 minutes after administration, comparing with placebo. No adverse events have been observed throughout the study. This research confirmed anti-inflammatory properties of nebulized apocynin, which might be an effective and safe drug in bronchial asthma.
哮喘是一种气道炎症性疾病，氧化应激已被证明参与其发病机制。阿朴吗啡通过阻断烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶的激活，有效抑制活性氧（ROS）的主要来源。本研究的目的是调查吸入阿朴吗啡对14名不吸烟轻度哮喘患者体内ROS和活性氮（RNS）浓度的影响。在雾化阿朴吗啡（0.5mg/mL）30、60和120分钟后，评估呼出气冷凝液（EBC）中的效果，并分析安全参数。与安慰剂相比，阿朴吗啡在60和120分钟后显著降低了EBC中的过氧化氢浓度。此外，与安慰剂相比，雾化后30和60分钟阿朴吗啡显著降低了NO(-2)浓度，给药后60和120分钟EBC中NO(-3)浓度显著降低。在整个研究过程中未观察到不良事件。本研究证实了雾化阿朴吗啡的抗炎特性，它可能是一种治疗支气管哮喘的有效且安全的药物。
