IVFAustralia, Sydney, NSW, Australia.
Am J Reprod Immunol. 2012 May;67(5):434-42. doi: 10.1111/j.1600-0897.2012.01105.x. Epub 2012 Feb 1.
To analyse the peripheral blood NK cells in women with repeated IVF failure (RIF) and a fertile control group to determine which parameters best differentiate the two populations.
Peripheral blood from the luteal phase of 171 women with RIF and 33 fertile controls was analysed by four-colour flow cytometry for NK cell concentration, subset differentiation and the activation marker CD69.
Women with RIF had significantly increased NK cell numbers as determined by concentration (P < 0.05) and percentage of lymphocytes (P < 0.001), increased concentration of the CD56(dim) subtype (P < 0.05), and increased concentration of activated CD56(dim) CD69(+) cells (P = 0.0001). There was no correlation between any NK cell parameters with the length of infertility or number of embryo transfer cycles.
Peripheral blood NK cell activity is significantly higher in women with RIF than in fertile controls. Future trials of immune therapy in women undergoing IVF should target those with high NK activity.
分析反复体外受精失败（RIF）女性和生育力正常对照组的外周血自然杀伤（NK）细胞，以确定哪些参数能最好地区分这两个群体。
采用四色流式细胞术分析 171 例 RIF 女性和 33 例生育力正常对照组患者黄体期外周血 NK 细胞浓度、亚群分化和激活标志物 CD69。
与生育力正常对照组相比，RIF 患者 NK 细胞数量（浓度：P<0.05；淋巴细胞比例：P<0.001）、CD56(dim)亚型浓度（P<0.05）、激活的 CD56(dim) CD69(+)细胞浓度（P=0.0001）均显著增加。任何 NK 细胞参数与不孕时间或胚胎移植周期数均无相关性。
与生育力正常对照组相比，RIF 患者外周血 NK 细胞活性显著升高。未来在接受 IVF 的女性中进行免疫治疗的试验应针对 NK 活性高的患者。
