Ghafourian Mehri, Karami Najmeh, Khodadadi Ali, Nikbakht Roshan
Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, e-mail:
Iran J Immunol. 2014 Jun;11(2):84-96.
Recurrent spontaneous abortion (RSA) and in vitro fertilization (IVF) failure with unknown causes are the controversial issues that are probably related to the immune system.
To compare circulating NK cells expressing activation and inhibition surface markers between patients with RSA and IVF failure with those of healthy multiparous and successful IVF control women, respectively.
In this case-control study peripheral blood samples were collected from 43 patients who included 23 women with RSA and 20 with IVF failure, plus 43 healthy control women comprising of 36 normal multiparous women and seven women with successful IVF. The expression of CD69, CD94 and CD161 surface markers on CD56+NK cells were assessed using specific monoclonal antibodies by flowcytometry.
The percentage of NK cells increased significantly in patients with RSA and in women with IVF failure in comparison to healthy multiparous and successful IVF control groups (p<0.001). The overall expression of CD69, CD94, CD161 were also increased significantly on NK cells in both patient groups compared to control groups (p<0.001).
Elevated expression of CD69 and CD161 on NK cells can be considered as immunological risk markers in RSA and IVF failure. However, it is not clear if high expression of CD94 on peripheral blood NK cells is related to abnormal activity of endometrial NK cells.
原因不明的复发性自然流产（RSA）和体外受精（IVF）失败是可能与免疫系统相关的有争议的问题。
分别比较RSA患者和IVF失败患者与健康经产妇及IVF成功的对照女性中表达激活和抑制表面标志物的循环NK细胞。
在这项病例对照研究中，收集了43名患者的外周血样本，其中包括23名RSA女性和20名IVF失败女性，另外还有43名健康对照女性，包括36名正常经产妇和7名IVF成功的女性。通过流式细胞术使用特异性单克隆抗体评估CD56 + NK细胞上CD69、CD94和CD161表面标志物的表达。
与健康经产妇和IVF成功的对照组相比，RSA患者和IVF失败女性中NK细胞的百分比显著增加（p<0.001）。与对照组相比，两个患者组中NK细胞上CD69、CD94、CD161的总体表达也显著增加（p<0.001）。
NK细胞上CD69和CD161的表达升高可被视为RSA和IVF失败中的免疫风险标志物。然而，外周血NK细胞上CD94的高表达是否与子宫内膜NK细胞的异常活性相关尚不清楚。
