Laboratory of Cell Biology and Histology, Science and Technology Faculty, University of the Basque Country, Sarriena z/g, Leioa, Basque Country, Spain.
Mar Pollut Bull. 2012 Mar;64(3):563-73. doi: 10.1016/j.marpolbul.2011.12.018. Epub 2012 Jan 31.
To assess the presence of endocrine disruptors in treated marine outfall discharges and their possible effects, mussels (Mytilus galloprovincialis) were caged in the environmental mixing zone of the outfall of the Santander sanitation system and in one control area. After 30, 60 and 90 days, samples were collected to perform chemical analyses (metals, anionic surfactants, alkylphenols, bisphenol A, phthalates and estrogenic hormones), biomarkers of general stress (lysosomal membrane stability-LMS, histopathology) and biomarkers of endocrine disruption (vitellogenin-like proteins and gonad index). There were no significant differences between outfall and control sites on contaminant levels, except for 4-tert-octylphenol which was higher in the outfall site. Bacteriological counts were higher in the outfall area. No relevant differences in biomarkers were detected between treated and control mussels. A significant reduction in LMS occurred in both groups after 90 days caging, indicating a stress situation possibly related to caging or to post-spawning reproductive state.
为了评估处理过的海洋排污口排放物中内分泌干扰物的存在及其可能的影响，贻贝（Mytilus galloprovincialis）被放置在桑坦德卫生系统排污口的环境混合区和一个对照区的笼子里。30、60 和 90 天后，采集样本进行化学分析（金属、阴离子表面活性剂、烷基酚、双酚 A、邻苯二甲酸酯和雌激素）、一般应激生物标志物（溶酶体膜稳定性-LMS、组织病理学）和内分泌干扰生物标志物（卵黄蛋白原样蛋白和性腺指数）。除了在排污口处含量较高的 4-叔辛基酚外，排污口和对照点的污染物水平没有显著差异。排污区的细菌计数较高。在处理过的和对照贻贝之间没有检测到生物标志物的相关差异。经过 90 天的笼养，两组的 LMS 均显著降低，表明可能与笼养或产卵后繁殖状态有关的应激情况。
