Ullah Anwar, Coronado Monika, Murakami Mário T, Betzel Christian, Arni Raghuvir K
Centro Multiusuário de Inovação Biomolecular, Departamento de Física, Universidade Estadual Paulista (UNESP), São José do Rio Preto-SP, Brazil.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1;68(Pt 2):211-3. doi: 10.1107/S1744309111054923. Epub 2012 Jan 26.
Snake-venom L-amino-acid oxidases (SV-LAAOs) trigger a wide range of local and systematic effects, including inhibition of platelet aggregation, cytotoxicity, haemolysis, apoptosis and haemorrhage. These effects mainly arise from the uncontrolled release of the hydrogen peroxide that is produced by the redox reaction involving L-amino acids catalyzed by these flavoenzymes. Taking their clinical relevance into account, few SV-LAAOs have been structurally characterized and the structural determinants responsible for their broad direct and indirect pharmacological activities remain unclear. In this work, an LAAO from Bothrops jararacussu venom (BJu-LAAO) was purified and crystallized. The BJu-LAAO crystals belonged to space group P2(1), with unit-cell parameters a = 66.38, b = 72.19, c = 101.53 Å, β = 90.9°. The asymmetric unit contained two molecules and the structure was determined and partially refined at 3.0 Å resolution.
蛇毒L-氨基酸氧化酶（SV-LAAOs）会引发一系列广泛的局部和全身效应，包括抑制血小板聚集、细胞毒性、溶血、细胞凋亡和出血。这些效应主要源于这些黄素酶催化的涉及L-氨基酸的氧化还原反应所产生的过氧化氢的失控释放。考虑到它们的临床相关性，很少有蛇毒L-氨基酸氧化酶的结构得到表征，并且负责其广泛直接和间接药理活性的结构决定因素仍不清楚。在这项工作中，从矛头蝮蛇毒中纯化并结晶出一种L-氨基酸氧化酶（BJu-LAAO）。BJu-LAAO晶体属于空间群P2(1)，晶胞参数为a = 66.38，b = 72.19，c = 101.53 Å，β = 90.9°。不对称单元包含两个分子，并且在3.0 Å分辨率下确定了结构并进行了部分精修。
