College of Plant Protection, Southwest University, Chongqing 400716, China.
J Econ Entomol. 2011 Dec;104(6):1892-9. doi: 10.1603/ec11095.
Transgenic rice expressing Bacillus thuringiensis (Bt) CrylAb protein is expected to be commercialized in China in the near future. The use of Bt rice for controlling insect pests sparks intensive debates regarding its biosafety. Folsomia candida is an euedaphic species and is often used as a "standard" test organism in assessing effects of environmental pollutants on soil organisms. In this study, growth, development, reproduction, and superoxide dismutase activity (SOD) of F. candida were investigated in the laboratory for populations reared on leaf tissue or leaf-soil mixtures of two CrylAb rice lines and a non-Bt rice isoline. Two independent tests were performed: 1) a 35-d test using petri dishes containing yeast diet (positive control) or fresh rice leaf tissue, and 2) a 28-d test in soil-litter microcosms containing yeast or a mixture of soil and rice leaf tissue. Biological parameters measured in both tests were number of progeny production, population growth rate, and SOD activity. For the petri dish test, data measured also included insect body length and number of exuviation. There were no significant differences between the populations reared on Bt and non-Bt rice leaf tissue in all measured parameters in both tests and for both Bt rice lines, suggesting no significant effects of the CrylAb protein in Bt rice on F. candida in the laboratory studies. Results of this study should add additional biosafety proofs for use of Bt rice to manage rice pests in China.
转 CrylAb 蛋白基因抗虫水稻预期在不久的将来在中国实现商业化。转 Bt 水稻用于防治虫害引起了激烈的争论，主要是关于其生物安全性。嗜卷书虱是一种土壤生物，通常被用作评估环境污染物对土壤生物影响的“标准”试验生物。在本研究中，我们在实验室中研究了两种转 CrylAb 水稻品系和一个非转 Bt 水稻对照品系的叶片组织或叶-土混合物对嗜卷书虱种群的生长、发育、繁殖和超氧化物歧化酶（SOD）活性的影响。进行了两项独立的测试：1）使用含有酵母饲料（阳性对照）或新鲜水稻叶片组织的培养皿进行的 35 天测试，和 2）在含有酵母或土壤和水稻叶片混合物的土壤-凋落物微宇宙中进行的 28 天测试。在这两项测试中测量的生物学参数包括后代的数量、种群增长率和 SOD 活性。对于培养皿测试，测量的数据还包括昆虫体长和蜕皮次数。在两项测试中，两种转 Bt 水稻品系的叶片组织上饲养的种群在所有测量参数上均无显著差异，这表明 CrylAb 蛋白在转 Bt 水稻中对嗜卷书虱没有显著影响。本研究的结果应为在中国利用转 Bt 水稻防治水稻害虫提供更多的生物安全证据。
