Rueanghiran Chalalai, Apiwathnasorn Chamnarn, Sangthong Pradit, Samung Yudthana, Ruangsittichai Jiraporn
Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2011 Nov;42(6):1381-7.
DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three amplicons the whole COI was obtained. Primer pair TY-J-1460/C1N2087 amplified 5' region of COI, LepF1/LepR1 the central and C1J2090/TL2N3014 the 3' region, generating COI amplicons of 650, 700 and 950 base pairs, respectively. When Ma. annulata sequences were compared with those from online sources, they formed a cluster group that is clearly distinct from other allied species.
基于DNA的鉴定系统利用线粒体细胞色素氧化酶亚基I（COI）基因，已能够验证某些分类群中的许多物种。这些引物组合能够在昆虫纲中普遍适用。在此，一组三对引物成功扩增了环斑曼蚊（Mansonia annulata）的COI，环斑曼蚊是马来布鲁线虫（Brugia malayi）的潜在传播媒介。通过合并所有三个扩增子，获得了完整的COI。引物对TY-J-1460/C1N2087扩增了COI的5'区域，LepF1/LepR1扩增了中间区域，C1J2090/TL2N3014扩增了3'区域，分别产生了650、700和950个碱基对的COI扩增子。当将环斑曼蚊的序列与来自在线来源的序列进行比较时，它们形成了一个聚类组，明显不同于其他近缘物种。
