Institute for Vector and Reservoir Control Research and Development, National Institute of Health Research and Development, the Ministry of Health of Indonesia, Salatiga, Indonesia.
HSM, University of Montpellier, CNRS, IRD, Montpellier, France.
Front Cell Infect Microbiol. 2021 Apr 21;11:631508. doi: 10.3389/fcimb.2021.631508. eCollection 2021.
Chikungunya is repeatedly affecting Indonesia through successive outbreaks. The Asian genotype has been present in Asia since the late 1950s while the ECSA-IOL (East/Central/South Africa - Indian Ocean Lineage) genotype invaded Asia in 2005. In order to determine the extension of the circulation of the chikungunya virus (CHIKV) in Indonesia, mosquitoes were collected in 28 different sites from 12 Indonesian provinces in 2016-2017. The E1 subunit of the CHIKV envelope gene was sequenced while mosquitoes were genotyped using the mitochondrial 1 (cytochrome C oxidase subunit 1) gene to determine whether a specific population was involved in the vectoring of CHIKV. A total of 37 CHIKV samples were found in 28 , 8 and 1 out of 15,362 samples collected and tested. These viruses, like all Indonesian CHIKV since 2000, belonged to a genotype we propose to call the Asian-Pacific genotype. It also comprises the Yap isolates and viruses having emerged in Polynesia, the Caribbean and South America. They differ from the CHIKV of the Asian genotype found earlier in Indonesia indicating a replacement. These results raise the question of the mechanisms behind this fast and massive replacement.
基孔肯雅热反复通过连续爆发影响印度尼西亚。亚洲基因型自 20 世纪 50 年代末以来一直在亚洲存在，而 ECSA-IOL（东/中非/南非-印度洋谱系）基因型于 2005 年入侵亚洲。为了确定基孔肯雅病毒（CHIKV）在印度尼西亚的传播范围，2016 年至 2017 年间，从印度尼西亚 12 个省的 28 个不同地点收集了蚊子。对 CHIKV 包膜基因的 E1 亚基进行了测序，同时使用线粒体 1（细胞色素 C 氧化酶亚基 1）基因对蚊子进行了基因分型，以确定特定种群是否参与了 CHIKV 的传播。在收集和检测的 15362 个样本中，共发现 28 个、8 个和 1 个样本中存在 37 个 CHIKV 样本。这些病毒与 2000 年以来所有印度尼西亚的 CHIKV 一样，属于我们提议称为亚洲-太平洋基因型的基因型。它还包括雅蒲岛分离株和在波利尼西亚、加勒比海和南美洲出现的病毒。它们与早些时候在印度尼西亚发现的亚洲基因型的 CHIKV 不同，表明已经发生了取代。这些结果提出了一个问题，即这种快速和大规模取代的背后机制是什么。
