[慢性阻塞性肺疾病患者血清中基质金属蛋白酶-9和金属蛋白酶组织抑制剂-1的浓度]

[Concentrations of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 in serum of patients with chronic obstructive pulmonary disease].

作者信息

Piesiak Paweł, Brzecka Anna, Kosacka Monika, Passowicz-Muszyńska Ewa, Dyła Tomasz, Jankowska Renata

机构信息

Akademia Medyczna we Wrocławiu, Klinika Pulmonologii i Nowotworów Płuc.

出版信息

Pol Merkur Lekarski. 2011 Nov;31(185):270-3.

PMID:22299526

Abstract

UNLABELLED

Chronic obstructive pulmonary disease (COPD) is characterized by the progressive destruction of the extracellular matrix of the lung. Matrix metalloproteinases (MMPs) and their inhibitors, especially tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), play a central role in the lung remodeling in COPD. Their concentrations in the sputum and bronchoalveolar lavage are considered as a sign of the local inflammation in COPD patients. The aim of the study was to evaluate of the MMP-9 and TIMP-1 serum concentration in COPD patients with respect to the correlation with a systemic inflammatory process.

MATERIAL AND METHODS

Thirty six COPD patients (29 male and 7 female) were enrolled into the study. Age-matched, healthy 15 subjects (11 male and 4 female) were selected as a control group. Serum levels of MMP-9, TIMP-1 and CRP were measured. The pre- and post-bronchodilator forced expiratory volume in one second (FEV1), forced vital capacity (FVC), and FEV1/FVC ratio were determined. The Spearman correlation was performed.

RESULTS

The serum MMP-9 concentration was significantly higher in the COPD patients than that in the control group (COPD: 194.8 +/- 129.1 ng x ml(-1); control subjects: 11.7 +/- 2.1 ng x ml(-1); p < 0.001). The serum concentration of CRP was significantly higher in the COPD patients than that in the control group (COPD: 14.3 +/- 6.3 ng x ml(-1); control subjects: 6.2 +/- 2.8 ng x ml(-1); p < 0.001). There was statistically significant correlation between the MMP-9 concentration and the CRP level in the serum of patients with COPD (p < 0.01; r = 0.45). There were no significant differences in the serum TIMP-1 concentrations between the control group and COPD patients. There was statistically significant, negative correlation between the FEV1 % of predicted value and the MMP-9 concentration (p = 0.03; r = -0.43).

CONCLUSIONS

MMP-9 may play an important role in the systemic inflammatory process in COPD. The serum concentrations of MMP-9 correlate with the stage of COPD.

摘要

未标注

慢性阻塞性肺疾病（COPD）的特征是肺细胞外基质的进行性破坏。基质金属蛋白酶（MMPs）及其抑制剂，尤其是基质金属蛋白酶-1组织抑制剂（TIMP-1），在COPD的肺重塑中起核心作用。它们在痰液和支气管肺泡灌洗中的浓度被视为COPD患者局部炎症的一个标志。本研究的目的是评估COPD患者血清中MMP-9和TIMP-1浓度与全身炎症过程的相关性。

材料与方法

36例COPD患者（29例男性和7例女性）纳入研究。选择15例年龄匹配的健康受试者（11例男性和4例女性）作为对照组。测量血清中MMP-9、TIMP-1和CRP的水平。测定支气管扩张剂使用前和使用后一秒用力呼气量（FEV1）、用力肺活量（FVC）以及FEV1/FVC比值。进行Spearman相关性分析。

结果

COPD患者血清MMP-9浓度显著高于对照组（COPD：194.8±129.1 ng·ml⁻¹；对照组受试者：11.7±2.1 ng·ml⁻¹；p<0.001）。COPD患者血清CRP浓度显著高于对照组（COPD：14.3±6.3 ng·ml⁻¹；对照组受试者：6.2±2.8 ng·ml⁻¹；p<0.001）。COPD患者血清中MMP-9浓度与CRP水平之间存在统计学显著相关性（p<0.01；r = 0.45）。对照组与COPD患者血清TIMP-1浓度无显著差异。预测值的FEV1%与MMP-9浓度之间存在统计学显著的负相关性（p = 0.03；r = -0.43）。