Kochetkova E A, Ugaĭ L G, Maĭstrovskaia Iu V, Buria K A, Nevzorova V A
Ter Arkh. 2012;84(8):37-40.
To determine a relationship between matrix metalloproteinase-9 (MMP-9) activity and its tissue inhibitors TIMP-1 and 2, tumor necrosis factor-alpha (TNF-alpha), bone mineral density (BMD), and bone exchange in chronic obstructive pulmonary disease (COPD).
Seventy-six patients with COPD and 20 healthy volunteers were examined using dual-energy X-ray absorptiometry n the lumbar spine (L(II)-L(IV)) and femoral neck (FN). The serum levels of MMP-9, TIMP-1 and TIMP-2, TNF-alpha, and beta-Crosslaps (betaCL) were measured.
There was a higher MMP-9 level in COPD than that in the controls ((383.8 +/- 54.2 and 137.6 +/- 31.4 pg/ml, respectively; p < 0.01). The levels of TIMP-1 and TIMP-2 were not different from those in the control group. An inverse correlation was found between forced expiratory volume in one second (FEV1) and MMP-9 concentration (r = -0.59; p = 0.002) and a positive correlation with smoking index (r = 0.47; p = 0.04). There was an inverse correlation between MMP-9 concentration and BMD in both L(II)-L(IV) and FN (r = -0.67; p < 0.001 and r = -0.61; p < 0.01, respectively) and a direct correlation with betaCL (r = 0.53; p = 0.04). An inverse correlation was established between TNF-alpha and T index in both L(II)-L(IV) (r = -0.54; p < 0.01) and FN (r = -0.48; p < 0.01). At the same time, the level of TNF-alpha directly correlated with the bone resorption marker betaCL (r = 0.53; p = 0.002) and MMP-9 (r = 0.57; p = 0.003).
Elevated MMP-9 levels may play an important role in type I collagen degradation, giving rise to enhanced bone resorption in COPD.
确定慢性阻塞性肺疾病（COPD）中基质金属蛋白酶-9（MMP-9）活性与其组织抑制剂TIMP-1和TIMP-2、肿瘤坏死因子-α（TNF-α）、骨密度（BMD）以及骨转换之间的关系。
对76例COPD患者和20名健康志愿者进行腰椎（L(II)-L(IV)）和股骨颈（FN）的双能X线吸收测定。检测血清中MMP-9、TIMP-1和TIMP-2、TNF-α以及β-交联C端肽（βCL）的水平。
COPD患者的MMP-9水平高于对照组（分别为(383.8±54.2和137.6± 31.4 pg/ml；p<0.01）。TIMP-1和TIMP-2的水平与对照组无差异。一秒用力呼气容积（FEV1）与MMP-9浓度呈负相关（r = -0.59；p = 0.002），与吸烟指数呈正相关（r = 0.47；p = 0.04）。MMP-9浓度与L(II)-L(IV)和FN的骨密度均呈负相关（分别为r = -0.67；p<0.001和r = -0.61；p<0.01），与βCL呈正相关（r = 0.53；p = 0.04）。TNF-α与L(II)-L(IV)（r = -0.54；p<0.01）和FN（r = -0.48；p<0.01）的T值均呈负相关。同时，TNF-α水平与骨吸收标志物βCL（r = 0.53；p = 0.002）和MMP-9（r = 0.57；p = 0.003）直接相关。
MMP-9水平升高可能在I型胶原降解中起重要作用，导致COPD患者骨吸收增强。
