定量实时 PCR 检测医院环境中鲍曼不动杆菌的定植情况。
Quantitative real-time PCR for detection of Acinetobacter baumannii colonization in the hospital environment.
机构信息
Unit of Infectious Diseases, Microbiology, and Preventive Medicine and Institute of Biomedicine of Sevilla (IBiS), University Hospital Virgen del Rocío/CSIC/University of Sevilla, Sevilla, Spain.
出版信息
J Clin Microbiol. 2012 Apr;50(4):1412-4. doi: 10.1128/JCM.06566-11. Epub 2012 Feb 1.
Abstract
A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria.
摘要
研究开发了一种实时 PCR 检测方法，用于检测医院设备上鲍曼不动杆菌的存在情况，并与 100 份医院环境样本的传统细菌培养进行了比较。与传统培养相比，实时 PCR 在 4 小时内即可检测到受污染的表面，具有较高的灵敏度（100%）。38%的样本实时 PCR 检测结果为阳性而细菌培养结果为阴性（假阳性），这可能表明存在大量与活细菌无关的细菌 DNA。
相似文献
1
Quantitative real-time PCR for detection of Acinetobacter baumannii colonization in the hospital environment.定量实时 PCR 检测医院环境中鲍曼不动杆菌的定植情况。
J Clin Microbiol. 2012 Apr;50(4):1412-4. doi: 10.1128/JCM.06566-11. Epub 2012 Feb 1.
2
Outbreak of multidrug-resistant Acinetobacter baumannii in an intensive care unit.重症监护病房中多重耐药鲍曼不动杆菌的暴发
New Microbiol. 2014 Apr;37(2):185-91. Epub 2014 Apr 1.
3
Nosocomial outbreak of infection with multidrug-resistant Acinetobacter baumannii in a medical center in Taiwan.台湾某医疗中心耐多药鲍曼不动杆菌的医院感染暴发。
Infect Control Hosp Epidemiol. 2009 Jan;30(1):34-8. doi: 10.1086/592704.
4
Epidemiology and resistance features of Acinetobacter baumannii isolates from the ward environment and patients in the burn ICU of a Chinese hospital.中国一家医院烧伤重症监护病房病房环境及患者中分离的鲍曼不动杆菌的流行病学和耐药特征
J Microbiol. 2016 Aug;54(8):551-8. doi: 10.1007/s12275-016-6146-0. Epub 2016 Aug 2.
5
Carbapenem-resistant Acinetobacter baumannii contamination in an intensive care unit.重症监护病房中耐碳青霉烯类鲍曼不动杆菌污染情况
Rev Soc Bras Med Trop. 2017 Mar-Apr;50(2):167-172. doi: 10.1590/0037-8682-0329-2016.
6
Comparison of OmpA Gene-Targeted Real-Time PCR with the Conventional Culture Method for Detection of Acinetobacter baumanii in Pneumonic BALB/c Mice.靶向OmpA基因的实时荧光定量PCR与传统培养方法检测肺炎性BALB/c小鼠鲍曼不动杆菌的比较
Iran Biomed J. 2019 Mar;23(2):159-64. doi: 10.29252/.23.2.159.
7
Characteristics of carbapenem-resistant Acinetobacter spp. other than Acinetobacter baumannii in South Korea.韩国除鲍曼不动杆菌外的碳青霉烯类耐药不动杆菌的特性。
Int J Antimicrob Agents. 2012 Jan;39(1):81-5. doi: 10.1016/j.ijantimicag.2011.08.006. Epub 2011 Oct 12.
8
Clinical validation of a real-time polymerase chain reaction assay for rapid detection of Acinetobacter baumannii colonization.一种用于快速检测鲍曼不动杆菌定植的实时聚合酶链反应检测方法的临床验证
J Hosp Infect. 2016 Sep;94(1):68-71. doi: 10.1016/j.jhin.2016.04.008. Epub 2016 Apr 23.
9
Spread of a single clone Acinetobacter baumannii strain in an intensive care unit of a teaching hospital in Turkey.一株鲍曼不动杆菌单一克隆菌株在土耳其一家教学医院重症监护病房的传播。
New Microbiol. 2005 Oct;28(4):337-43.
10
Study on the resistant genes to carbapenems and epidemiological characterization of multidrug-resistant Acinetobacter baumannii isolates.碳青霉烯类耐药基因研究与多重耐药鲍曼不动杆菌的流行病学特征。
Microb Drug Resist. 2013 Apr;19(2):117-23. doi: 10.1089/mdr.2012.0049. Epub 2012 Jul 30.
引用本文的文献
1
Microbial risks in drinking water systems: persistence and public health implications of opportunistic premise plumbing pathogens.饮用水系统中的微生物风险：机会性室内管道病原体的持久性及对公众健康的影响
Front Microbiol. 2025 May 8;16:1575789. doi: 10.3389/fmicb.2025.1575789. eCollection 2025.
2
Development and validation of multiplex real-time PCR for simultaneous detection of six bacterial pathogens causing lower respiratory tract infections and antimicrobial resistance genes.建立并验证多重实时 PCR 法同时检测六种引起下呼吸道感染的细菌病原体及其耐药基因
BMC Infect Dis. 2024 Feb 7;24(1):164. doi: 10.1186/s12879-024-09028-2.
3
Sub-minimum inhibitory concentrations (sub-MICs) of colistin on biofilm formation potency, adherence, and invasion to epithelial host cells: an experimental study in an Iranian children's referral hospital.多粘菌素亚最小抑菌浓度（sub-MICs）对生物膜形成能力、黏附及侵袭上皮宿主细胞的影响：伊朗一家儿童医院的实验研究。
Microbiol Spectr. 2024 Feb 6;12(2):e0252323. doi: 10.1128/spectrum.02523-23. Epub 2024 Jan 17.
4
Photodynamic Therapy and Its Synergism with Melittin Against Drug-Resistant Acinetobacter baumannii Isolates with High Biofilm Formation Ability.光动力疗法及其与蜂毒素联用对高生物膜形成能力耐药鲍曼不动杆菌的协同作用。
Curr Microbiol. 2023 Aug 18;80(10):324. doi: 10.1007/s00284-023-03356-3.
5
Development of diagnostic tests for pathogen identification and detection of antimicrobial resistance on WHO global priority pathogens using modular real-time nucleic acid amplification test.利用模块化实时核酸扩增检测技术，针对世卫组织全球优先病原体，开发病原体鉴定诊断检测和抗微生物药物耐药性检测方法。
Int Microbiol. 2023 Aug;26(3):563-577. doi: 10.1007/s10123-023-00321-9. Epub 2023 Jan 17.
6
The effects of antidepressants fluoxetine, sertraline, and amitriptyline on the development of antibiotic resistance in Acinetobacter baumannii.抗抑郁药氟西汀、舍曲林和阿米替林对鲍曼不动杆菌抗生素耐药性发展的影响。
Arch Microbiol. 2022 Mar 30;204(4):230. doi: 10.1007/s00203-022-02853-6.
7
Duplex dPCR System for Rapid Identification of Gram-Negative Pathogens in the Blood of Patients with Bloodstream Infection: A Culture-Independent Approach.用于快速鉴定血流感染患者血液中革兰氏阴性病原体的双重实时 PCR 系统：一种非培养依赖的方法。
J Microbiol Biotechnol. 2021 Nov 28;31(11):1481-1489. doi: 10.4014/jmb.2103.03044.
8
Detection of gene in carbapenem-resistant strains and targeting with biocompounds.耐碳青霉烯类菌株中基因的检测及生物化合物靶向作用
Iran J Basic Med Sci. 2021 May;24(5):690-698. doi: 10.22038/IJBMS.2021.52852.11917.
9
Control of outbreak in the neonatal intensive care unit in Latvia: whole-genome sequencing powered investigation and closure of the ward.拉脱维亚新生儿重症监护病房暴发疫情的控制：全基因组测序助力调查和关闭病房。
Antimicrob Resist Infect Control. 2019 May 22;8:84. doi: 10.1186/s13756-019-0537-z. eCollection 2019.
10
Development of a TaqMan Array card to target 21 purulent meningitis-related pathogens.开发一种 TaqMan 阵列卡，以针对 21 种化脓性脑膜炎相关病原体。
BMC Infect Dis. 2019 Mar 28;19(1):289. doi: 10.1186/s12879-019-3856-z.
本文引用的文献
1
Positive predictive value of Leeds acinetobacter medium for environmental surveillance of Acinetobacter baumannii.利兹不动杆菌培养基对鲍曼不动杆菌环境监测的阳性预测值。
J Clin Microbiol. 2011 Dec;49(12):4416. doi: 10.1128/JCM.05412-11. Epub 2011 Sep 28.
2
Long-term control of hospital-wide, endemic multidrug-resistant Acinetobacter baumannii through a comprehensive "bundle" approach.通过综合“捆绑”方法实现医院范围内地方性多重耐药鲍曼不动杆菌的长期控制。
Am J Infect Control. 2009 Nov;37(9):715-22. doi: 10.1016/j.ajic.2009.01.008. Epub 2009 May 19.
3
Checklist for optimization and validation of real-time PCR assays.实时荧光定量PCR检测方法的优化与验证清单。
J Clin Lab Anal. 2009;23(3):145-51. doi: 10.1002/jcla.20307.
4
Molecular epidemiology of Acinetobacter baumannii spread in an adult intensive care unit under an endemic setting.鲍曼不动杆菌在地方流行环境下于成人重症监护病房传播的分子流行病学研究
Am J Infect Control. 2008 Aug;36(6):444-52. doi: 10.1016/j.ajic.2007.09.010.
5
Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options.鲍曼不动杆菌：流行病学、抗菌药物耐药性及治疗选择
Clin Infect Dis. 2008 Apr 15;46(8):1254-63. doi: 10.1086/529198.
6
Real-time PCR in clinical microbiology: applications for routine laboratory testing.临床微生物学中的实时聚合酶链反应：在常规实验室检测中的应用
Clin Microbiol Rev. 2006 Jan;19(1):165-256. doi: 10.1128/CMR.19.1.165-256.2006.
7
An outbreak of multidrug-resistant Acinetobacter baumannii associated with pulsatile lavage wound treatment.一起与脉动冲洗伤口治疗相关的多重耐药鲍曼不动杆菌暴发。
JAMA. 2004 Dec 22;292(24):3006-11. doi: 10.1001/jama.292.24.3006.
8
Surveillance of an adult intensive care unit for long-term persistence of a multi-resistant strain of Acinetobacter baumannii.
Eur J Clin Microbiol Infect Dis. 1998 Mar;17(3):171-6. doi: 10.1007/BF01691113.
9
Description of Leeds Acinetobacter Medium, a new selective and differential medium for isolation of clinically important Acinetobacter spp., and comparison with Herellea agar and Holton's agar.利兹不动杆菌培养基的描述，一种用于分离临床重要不动杆菌属的新型选择性和鉴别性培养基，以及与赫雷拉琼脂和霍尔顿琼脂的比较。
J Clin Microbiol. 1994 Oct;32(10):2353-8. doi: 10.1128/jcm.32.10.2353-2358.1994.
Zotero 插件