Unit of Infectious Diseases, Microbiology, and Preventive Medicine and Institute of Biomedicine of Sevilla (IBiS), University Hospital Virgen del Rocío/CSIC/University of Sevilla, Sevilla, Spain.
J Clin Microbiol. 2012 Apr;50(4):1412-4. doi: 10.1128/JCM.06566-11. Epub 2012 Feb 1.
A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria.
研究开发了一种实时 PCR 检测方法，用于检测医院设备上鲍曼不动杆菌的存在情况，并与 100 份医院环境样本的传统细菌培养进行了比较。与传统培养相比，实时 PCR 在 4 小时内即可检测到受污染的表面，具有较高的灵敏度（100%）。38%的样本实时 PCR 检测结果为阳性而细菌培养结果为阴性（假阳性），这可能表明存在大量与活细菌无关的细菌 DNA。
