Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ankara University, 06560, Yenimahalle, Ankara, Turkey.
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
Arch Microbiol. 2022 Mar 30;204(4):230. doi: 10.1007/s00203-022-02853-6.
This study investigates the effects of antidepressants fluoxetine, sertraline, and amitriptyline on the development of antibiotic resistance in clinical Acinetobacter baumannii isolates. The isolates were exposed to fluoxetine, sertraline, and amitriptyline for 30 days, respectively. The bacteria that developed resistance to gentamicin, imipenem, colistin, and ciprofloxacin were isolated and expression levels of some antibiotic-resistance genes were determined by quantitative reverse-transcriptase PCR. Before and after the exposure, minimum inhibitory concentration (MIC) values of the bacteria were determined by the microdilution method. The statistical analysis was performed using Student's t test. A time-dependent increase was observed in the number of bacteria that developed resistance and increased the MIC value. After exposure to fluoxetine and sertraline, decreases were observed for efflux and outer membrane porin genes in isolates that developed colistin resistance, and increases were observed in isolates that developed ciprofloxacin resistance. These observations suggest that these antidepressants have similar effects on the development of resistance. While the exposure to fluoxetine did not result in the development of resistance to imipenem, it was observed after exposure to sertraline and amitriptyline, and a common decrease in ompA gene expression was determined in these isolates. To our knowledge, the comparative effects of selected antidepressants on the development of antibiotic resistance in A. baumannii are reported and presented in the literature here for the first time.
本研究探讨了抗抑郁药氟西汀、舍曲林和阿米替林对临床鲍曼不动杆菌分离株抗生素耐药性发展的影响。将分离株分别暴露于氟西汀、舍曲林和阿米替林 30 天。分离出对庆大霉素、亚胺培南、多粘菌素和环丙沙星耐药的细菌，并通过定量逆转录聚合酶链反应测定一些抗生素耐药基因的表达水平。通过微量稀释法测定细菌的最小抑菌浓度（MIC）值。使用学生 t 检验进行统计分析。观察到随着时间的推移，耐药细菌的数量增加，MIC 值增加。在暴露于氟西汀和舍曲林后，对多粘菌素耐药分离株的外排和外膜孔蛋白基因的表达减少，而对环丙沙星耐药分离株的表达增加。这些观察结果表明，这些抗抑郁药对耐药性的发展有相似的影响。虽然氟西汀暴露不会导致对亚胺培南产生耐药性，但在暴露于舍曲林和阿米替林后观察到，这些分离株的 ompA 基因表达普遍下降。据我们所知，本文首次在文献中报道并介绍了选定的抗抑郁药对鲍曼不动杆菌抗生素耐药性发展的比较影响。
