Webster H V, Bone A J, Webster K A, Wilkin T J
Endocrine Section, Medicine II, Southampton General Hospital, U.K.
J Immunol Methods. 1990 Nov 6;134(1):95-100. doi: 10.1016/0022-1759(90)90116-d.
A recently developed competitive enzyme-linked immunosorbent assay (ELISA) was compared with a conventional competitive radioimmunoassay (RIA) for the measurement of rat insulin in culture medium. Fifty-six samples were analysed by both assays. There was a correlation coefficient of r = 0.783 between results obtained using the two assay systems. The binding curves of the two assays were differently shaped, so that the ELISA gave good reproducibility over the concentration range 5-50 microU/ml insulin with inter- and intra-assay coefficients of variation less than 14%, but poor reproducibility at higher concentrations. Conversely, the RIA showed excellent reproducibility at concentrations greater than 50 microU/ml insulin, but poor sensitivity and high coefficients of variation below this level. The ELISA procedure offers practical advantages over the RIA, and performs well when measuring physiological concentrations of insulin.
一种最近开发的竞争性酶联免疫吸附测定法（ELISA）与传统的竞争性放射免疫测定法（RIA）被用于比较测定培养基中的大鼠胰岛素。两种测定法对56个样本进行了分析。使用这两种测定系统获得的结果之间的相关系数r = 0.783。两种测定法的结合曲线形状不同，因此ELISA在胰岛素浓度范围为5 - 50微单位/毫升时具有良好的重复性，批间和批内变异系数小于14%，但在较高浓度时重复性较差。相反，RIA在胰岛素浓度大于50微单位/毫升时显示出优异的重复性，但在此浓度以下灵敏度较差且变异系数较高。ELISA方法相对于RIA具有实际优势，并且在测量生理浓度的胰岛素时表现良好。
