通过添加Rv3020c衍生肽改进用于牛结核病鉴别诊断的皮肤试验。
Improved skin test for differential diagnosis of bovine tuberculosis by the addition of Rv3020c-derived peptides.
作者信息
Jones Gareth J, Whelan Adam, Clifford Derek, Coad Mick, Vordermeier H Martin
机构信息
TB Research Group, Animal Health and Veterinary Laboratories Agency, New Haw, Addlestone, Surrey, United Kingdom.
出版信息
Clin Vaccine Immunol. 2012 Apr;19(4):620-2. doi: 10.1128/CVI.00024-12. Epub 2012 Feb 1.
Abstract
A peptide cocktail derived from the mycobacterial antigens ESAT-6, CFP-10, and Rv3615c allowed differentiation between Mycobacterium bovis-infected and M. bovis bacillus Calmette-Guérin (BCG)-vaccinated cattle when used as a skin test reagent for a "DIVA" test (i.e., a test capable of differentiating infected and uninfected vaccinated animals). Addition of the antigen Rv3020c improves the diagnostic sensitivity without compromising specificity in the face of BCG or Johne's disease vaccination.
摘要
一种源自分枝杆菌抗原ESAT-6、CFP-10和Rv3615c的肽混合物，当用作“鉴别感染与疫苗接种动物”（DIVA）试验（即一种能够区分感染动物和未感染的接种疫苗动物的试验）的皮肤试验试剂时，可区分感染牛分枝杆菌的牛和接种卡介苗（BCG）的牛。添加抗原Rv3020c可提高诊断敏感性，同时在面对卡介苗接种或副结核菌素病疫苗接种时不影响特异性。
相似文献
1
Improved skin test for differential diagnosis of bovine tuberculosis by the addition of Rv3020c-derived peptides.通过添加Rv3020c衍生肽改进用于牛结核病鉴别诊断的皮肤试验。
Clin Vaccine Immunol. 2012 Apr;19(4):620-2. doi: 10.1128/CVI.00024-12. Epub 2012 Feb 1.
2
Development of a skin test for bovine tuberculosis for differentiating infected from vaccinated animals.牛结核病感染与免疫动物鉴别用皮试的研制。
J Clin Microbiol. 2010 Sep;48(9):3176-81. doi: 10.1128/JCM.00420-10. Epub 2010 Jun 30.
3
Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in cattle.使用源自抗原ESAT-6和CFP-10的合成肽对牛结核病进行牛的鉴别诊断。
Clin Diagn Lab Immunol. 2001 May;8(3):571-8. doi: 10.1128/CDLI.8.3.571-578.2001.
4
Screening of predicted secreted antigens from Mycobacterium bovis identifies potential novel differential diagnostic reagents.牛分枝杆菌预测分泌抗原的筛选鉴定出潜在的新型鉴别诊断试剂。
Clin Vaccine Immunol. 2010 Sep;17(9):1344-8. doi: 10.1128/CVI.00261-10. Epub 2010 Jul 28.
5
Antigen mining to define Mycobacterium bovis antigens for the differential diagnosis of vaccinated and infected animals: A VLA perspective.通过抗原挖掘确定用于区分接种和感染动物的牛分枝杆菌抗原：英国兽医实验室局的观点
Transbound Emerg Dis. 2009 Aug;56(6-7):240-7. doi: 10.1111/j.1865-1682.2009.01070.x. Epub 2009 Apr 23.
6
Assessment of a protein cocktail-based skin test for bovine tuberculosis in a double-blind field test in cattle.在牛的双盲现场试验中对基于蛋白混合物的牛结核病皮肤试验的评估。
Clin Vaccine Immunol. 2013 Apr;20(4):482-90. doi: 10.1128/CVI.00592-12. Epub 2013 Jan 30.
7
Use of antigen-specific interleukin-2 to differentiate between cattle vaccinated with Mycobacterium bovis BCG and cattle infected with M. bovis.使用抗原特异性白细胞介素-2区分接种卡介苗的牛和感染牛分枝杆菌的牛。
Clin Vaccine Immunol. 2014 Jan;21(1):39-45. doi: 10.1128/CVI.00522-13. Epub 2013 Oct 30.
8
Display of Antigens on Polyester Inclusions Lowers the Antigen Concentration Required for a Bovine Tuberculosis Skin Test.聚酯包涵体上抗原的展示降低了牛结核病皮肤试验所需的抗原浓度。
Clin Vaccine Immunol. 2015 Oct 28;23(1):19-26. doi: 10.1128/CVI.00462-15. Print 2016 Jan.
9
Test performance data demonstrates utility of a cattle DIVA skin test reagent (DST-F) compatible with BCG vaccination.检测性能数据表明，与卡介苗（BCG）接种兼容的牛 DIVA 皮肤测试试剂（DST-F）具有实用性。
Sci Rep. 2022 Jul 14;12(1):12052. doi: 10.1038/s41598-022-16092-8.
10
Field evaluation of specific mycobacterial protein-based skin test for the differentiation of Mycobacterium bovis-infected and Bacillus Calmette Guerin-vaccinated crossbred cattle in Ethiopia.在埃塞俄比亚，对基于特定分枝杆菌蛋白的皮肤试验进行现场评估，以区分感染牛分枝杆菌和接种卡介苗的杂交牛。
Transbound Emerg Dis. 2022 Jul;69(4):e1-e9. doi: 10.1111/tbed.14252. Epub 2021 Aug 19.
引用本文的文献
1
Effects of different vaccination regimes on the immunodiagnosis of tuberculosis in goats and evaluation of defined antigens.不同疫苗接种方案对山羊结核病免疫诊断的影响及特定抗原的评估
Front Vet Sci. 2025 Jan 15;11:1524461. doi: 10.3389/fvets.2024.1524461. eCollection 2024.
2
Comparative performance of tuberculin and defined-antigen cocktails for detecting bovine tuberculosis in BCG-vaccinated cattle in natural settings.结核菌素与特定抗原组合在自然环境中检测卡介苗接种牛群中牛结核病的比较性能
Sci Rep. 2025 Feb 7;15(1):4564. doi: 10.1038/s41598-025-85389-1.
3
Orally administered live BCG and heat-inactivated Mycobacterium bovis protect bison against experimental bovine tuberculosis.口服活卡介苗和热灭活牛分枝杆菌可保护野牛免受实验性牛结核病的侵害。
Sci Rep. 2025 Jan 30;15(1):3764. doi: 10.1038/s41598-025-88176-0.
4
The Rv3874-Rv3875 chimeric protein shows a promiscuous serodiagnostic potential for tuberculosis.Rv3874-Rv3875 嵌合蛋白显示出对结核病有混杂的血清学诊断潜力。
Tuberculosis (Edinb). 2022 Sep;136:102253. doi: 10.1016/j.tube.2022.102253. Epub 2022 Aug 30.
5
Field evaluation of specific mycobacterial protein-based skin test for the differentiation of Mycobacterium bovis-infected and Bacillus Calmette Guerin-vaccinated crossbred cattle in Ethiopia.在埃塞俄比亚，对基于特定分枝杆菌蛋白的皮肤试验进行现场评估，以区分感染牛分枝杆菌和接种卡介苗的杂交牛。
Transbound Emerg Dis. 2022 Jul;69(4):e1-e9. doi: 10.1111/tbed.14252. Epub 2021 Aug 19.
6
HIV-Infected Patients Developing Tuberculosis Disease Show Early Changes in the Immune Response to Novel Antigens.感染 HIV 的结核病患者表现出对新型抗原免疫反应的早期变化。
Front Immunol. 2021 Mar 12;12:620622. doi: 10.3389/fimmu.2021.620622. eCollection 2021.
7
A molecularly defined skin test reagent for the diagnosis of bovine tuberculosis compatible with vaccination against Johne's Disease.一种分子定义的皮肤测试试剂，用于诊断牛结核病，与针对约翰氏病的疫苗接种兼容。
Sci Rep. 2021 Feb 3;11(1):2929. doi: 10.1038/s41598-021-82434-7.
8
Immunogenicity and Protection against Challenge in Goats Vaccinated with BCG and Revaccinated after One Year.卡介苗接种山羊及一年后再次接种后的免疫原性和攻毒保护作用
Vaccines (Basel). 2020 Dec 10;8(4):751. doi: 10.3390/vaccines8040751.
9
Association of immune responses of Zebu and Holstein-Friesian cattle and resistance to mycobacteria in a BCG challenge model.Zebu 和荷斯坦-弗里森牛的免疫反应与卡介苗挑战模型中对分枝杆菌的抗性的相关性。
Transbound Emerg Dis. 2021 Nov;68(6):3360-3365. doi: 10.1111/tbed.13939. Epub 2020 Dec 14.
10
complex in wildlife: Review of current applications of antemortem and postmortem diagnosis.野生动物疾病复杂性：生前与死后诊断当前应用综述
Vet World. 2020 Sep;13(9):1822-1836. doi: 10.14202/vetworld.2020.1822-1836. Epub 2020 Sep 9.
本文引用的文献
1
Rv3615c is a highly immunodominant RD1 (Region of Difference 1)-dependent secreted antigen specific for Mycobacterium tuberculosis infection.Rv3615c 是一种高度免疫显性 RD1（差异区 1）依赖性分泌抗原，针对结核分枝杆菌感染具有特异性。
Proc Natl Acad Sci U S A. 2011 Apr 5;108(14):5730-5. doi: 10.1073/pnas.1015153108. Epub 2011 Mar 22.
2
Screening of predicted secreted antigens from Mycobacterium bovis identifies potential novel differential diagnostic reagents.牛分枝杆菌预测分泌抗原的筛选鉴定出潜在的新型鉴别诊断试剂。
Clin Vaccine Immunol. 2010 Sep;17(9):1344-8. doi: 10.1128/CVI.00261-10. Epub 2010 Jul 28.
3
Development of a skin test for bovine tuberculosis for differentiating infected from vaccinated animals.牛结核病感染与免疫动物鉴别用皮试的研制。
J Clin Microbiol. 2010 Sep;48(9):3176-81. doi: 10.1128/JCM.00420-10. Epub 2010 Jun 30.
4
Viral booster vaccines improve Mycobacterium bovis BCG-induced protection against bovine tuberculosis.病毒加强疫苗可增强卡介苗诱导的针对牛结核病的保护作用。
Infect Immun. 2009 Aug;77(8):3364-73. doi: 10.1128/IAI.00287-09. Epub 2009 Jun 1.
5
Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay.副结核病对使用γ-干扰素检测法诊断混合感染牛群中牛结核病的影响。
Vet Microbiol. 2009 Mar 30;135(3-4):389-93. doi: 10.1016/j.vetmic.2008.09.060. Epub 2008 Sep 21.
6
Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex.对高表达分枝杆菌基因的筛选确定Rv3615c是结核分枝杆菌复合群的一种有用的鉴别诊断抗原。
Infect Immun. 2008 Sep;76(9):3932-9. doi: 10.1128/IAI.00150-08. Epub 2008 Jun 2.
7
Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection.副结核病对自然混合感染山羊群中结核病诊断的干扰。
Vet Microbiol. 2008 Apr 1;128(1-2):72-80. doi: 10.1016/j.vetmic.2007.08.034. Epub 2007 Sep 18.
8
Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis.牛分枝杆菌和副结核分枝杆菌亚种共同感染的牛群中牛结核病根除诊断工具的评估
Vet Res. 2006 Jul-Aug;37(4):593-606. doi: 10.1051/vetres:2006021. Epub 2006 May 16.
9
Immune responses induced in cattle by vaccination with a recombinant adenovirus expressing Mycobacterial antigen 85A and Mycobacterium bovis BCG.用表达分枝杆菌抗原85A的重组腺病毒和牛分枝杆菌卡介苗对牛进行疫苗接种所诱导的免疫反应。
Infect Immun. 2006 Feb;74(2):1416-8. doi: 10.1128/IAI.74.2.1416-1418.2006.
10
Bovine NK cells can produce gamma interferon in response to the secreted mycobacterial proteins ESAT-6 and MPP14 but not in response to MPB70.牛自然杀伤细胞可对分泌型分枝杆菌蛋白ESAT-6和MPP14产生γ干扰素应答，但对MPB70无应答。
Infect Immun. 2005 Sep;73(9):5628-35. doi: 10.1128/IAI.73.9.5628-5635.2005.
Zotero 插件