硫醇-烯反应：一种用于在蛋白质上进行位点特异性标记的多功能工具，使用化学惰性标签进行顺磁 NMR。
Thiol-ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR.
机构信息
State-Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin, 300071, China.
出版信息
Chem Commun (Camb). 2012 Mar 11;48(21):2704-6. doi: 10.1039/c2cc17900h. Epub 2012 Feb 3.
PMID:22302218
Abstract
Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags.
摘要
通过 NMR 光谱学，将顺磁镧系元素特异性地标记在蛋白质上，可以为结构生物学提供有价值的长程结构约束。我们表明，硫醇-烯加成反应为通过化学稳定的方式用非常小的镧系元素标签标记蛋白质提供了一种强大的工具。
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