Hansen M S, Bang N U
Mol Cell Biochem. 1979 Apr 2;24(3):143-58. doi: 10.1007/BF00220733.
This reviews summarizes our evidence suggesting that the plasma protein enviroment influences platelet aggregation potential and metabolic activity. Cationic proteins are capable of restoring the aggreation potential of washed human platelets. The aggregation restoring effect of gamma globulin is inhibited by more anionic proteins in subfractions of Cohn fraction IV and fractions V and VI. Artificial enhancement of the net negative charge of plasma proteins through acylation produces derivatives capable of inhibiting platelet rich plasma. The oxygen consumption of washed human platelets is lower than in platelet rich plasma while the lactate production is identical. Autologus plasma, albumin or IgG immunoglobulin restores the oxygen consumption of washed platelets to values comparable to those obtained for platelet rich plasma, while the lactate production is unaffected. Fibrinogen on IgA myeloma protein increases the lactate production, but not the oxygen consumption. Cyclic AMP levels are considerably lower in washed platelets than in platelet rich plasma. Gamma globulin and albumin causes a futher decrease, which is progressive with time. Fibrinogen causes no change in platelet cyclic AMP content. It is suggested that these observations may in part be explained by the equilibriun between anionic and cationic proteins in the platelet microenvironment. This hypothesis appears applicable in certain situations.
本综述总结了我们的证据，表明血浆蛋白环境会影响血小板聚集潜能和代谢活性。阳离子蛋白能够恢复洗涤过的人血小板的聚集潜能。γ球蛋白的聚集恢复作用在科恩IV组分及V和VI组分的亚组分中会被更多阴离子蛋白抑制。通过酰化人工增强血浆蛋白的净负电荷会产生能够抑制富含血小板血浆的衍生物。洗涤过的人血小板的耗氧量低于富含血小板血浆中的耗氧量，而乳酸生成量相同。自体血浆、白蛋白或IgG免疫球蛋白可将洗涤过的血小板的耗氧量恢复到与富含血小板血浆相当的值，而乳酸生成不受影响。纤维蛋白原或IgA骨髓瘤蛋白会增加乳酸生成，但不增加耗氧量。洗涤过的血小板中的环磷酸腺苷（cAMP）水平显著低于富含血小板血浆中的水平。γ球蛋白和白蛋白会导致进一步降低，且随时间逐渐下降。纤维蛋白原不会改变血小板cAMP含量。有人认为，这些观察结果部分可由血小板微环境中阴离子和阳离子蛋白之间的平衡来解释。这一假说在某些情况下似乎适用。
