Meyer R D, Moudgil T, Detels R, Phair J P, Hirsch M S, Ho D D
Department of Medicine, University of California School of Medicine, Los Angeles 90048.
J Infect Dis. 1990 Dec;162(6):1370-2. doi: 10.1093/infdis/162.6.1370.
This study sought to define the seroprevalence of human T cell leukemia virus (HTLV) types I and II in selected populations of homosexual men. Serum specimens were screened for antibodies to HTLV and to human immunodeficiency virus (HIV) by enzyme immunoassay; successive testing of specimens with positive results was done by Western blotting and radioimmunoprecipitation assay (RIPA) and then by polymerase chain reaction (PCR) assay on available peripheral blood mononuclear cells (PBMC). Of 1290 specimens, only 4 had antibodies against HTLV confirmed by RIPA. PCR analysis of DNA from PBMC from two subjects showed one to be HTLV-I and the other to be HTLV-II; both men also had HIV antibodies. These results demonstrate a lower seroprevalence rate for HTLV than some previous studies and emphasize the need for specific confirmatory tests.
本研究旨在确定特定同性恋男性人群中I型和II型人类T细胞白血病病毒（HTLV）的血清流行率。通过酶免疫测定法对血清标本进行HTLV抗体和人类免疫缺陷病毒（HIV）抗体筛查；对检测结果呈阳性的标本依次采用蛋白质印迹法和放射免疫沉淀测定法（RIPA）进行检测，然后对可用的外周血单个核细胞（PBMC）进行聚合酶链反应（PCR）检测。在1290份标本中，只有4份经RIPA确认存在抗HTLV抗体。对两名受试者PBMC的DNA进行PCR分析显示，其中一人感染HTLV-I，另一人感染HTLV-II；这两名男性均同时存在HIV抗体。这些结果表明，HTLV的血清流行率低于此前的一些研究，并强调了进行特异性确证检测的必要性。
