Medical Research Council Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, United Kingdom.
J Neurosci. 2012 Feb 1;32(5):1867-74. doi: 10.1523/JNEUROSCI.5181-11.2012.
Mature presynaptic terminals release neurotransmitter both in response to activity and spontaneously. We found that axons of rat hippocampal neurons initially show very high levels of exclusively spontaneous release, which progressively switches over to the mature phenotype during synapse formation. These two modes of vesicle cycling derive from distinct pools throughout development and the initiation of activity-dependent release was independent of postsynaptic contacts, suggesting it is an autonomous presynaptic event.
成熟的突触前末梢既能对活动作出反应,也能自发释放神经递质。我们发现,大鼠海马神经元的轴突最初表现出极高水平的仅自发释放,这种释放在突触形成过程中逐渐转变为成熟表型。这两种囊泡循环模式源自整个发育过程中的不同池,并且依赖于活动的释放的起始与突触后接触无关,这表明它是一个自主的突触前事件。