Viswanathaswamy A H M, Koti B C, Gore Aparna, Thippeswamy A H M, Kulkarni R V
Department of Pharmacology, KLE University College of Pharmacy, Vidyanagar, Hubli-580 031, India.
Indian J Pharm Sci. 2011 Mar;73(2):139-45. doi: 10.4103/0250-474x.91572.
The present study was undertaken to investigate the antihyperglycemic and antihyperlipidemic effects of ethanol extract of Plectranthus amboinicus in normal and alloxan-induced diabetic rats. Diabetes was induced in Wistar rats by single intraperitoneal administration of alloxan monohydrate (150 mg/kg). Normal as well as diabetic rats were divided into groups (n=6) receiving different treatments. Graded doses (200 mg/kg and 400 mg/kg) of ethanol extract of Plectranthus amboinicus were studied in both normal and alloxan-induced diabetic rats for a period of 15 days. Glibenclamide (600 μg/kg) was used as a reference drug. Oral administration with graded doses of ethanol extract of Plectranthus amboinicus exhibited hypoglycemic effect in normal rats and significantly reduced the peak glucose levels after 120 min of glucose loading. In alloxan-induced diabetic rats, the daily oral treatment with ethanol extract of Plectranthus amboinicus showed a significant reduction in blood glucose. Besides, administration of ethanol extract of Plectranthus amboinicus for 15 days significantly decreased serum contents of total cholesterol, triglycerides whereas HDL-cholesterol, total proteins and calcium were effectively increased. Furthermore, effect of ethanol extract of Plectranthus amboinicus showed profound elevation of serum amylase and reduction of serum lipase. Histology examination showed ethanol extract of Plectranthus amboinicus exhibited almost normalization of damaged pancreatic architecture in rats with diabetes mellitus. Studies clearly demonstrated that ethanol extract of Plectranthus amboinicus leaves possesses hypoglycemic and antihyperlipidemic effects mediated through the restoration of the functions of pancreatic tissues and insulinotropic effect.
本研究旨在探讨安博因香茶菜乙醇提取物对正常及四氧嘧啶诱导的糖尿病大鼠的降血糖和降血脂作用。通过单次腹腔注射一水合四氧嘧啶（150 mg/kg）诱导Wistar大鼠患糖尿病。将正常大鼠和糖尿病大鼠分成若干组（n = 6）接受不同治疗。对正常及四氧嘧啶诱导的糖尿病大鼠研究了安博因香茶菜乙醇提取物的分级剂量（200 mg/kg和400 mg/kg），为期15天。格列本脲（600 μg/kg）用作参比药物。口服分级剂量的安博因香茶菜乙醇提取物在正常大鼠中表现出降血糖作用，并在葡萄糖负荷120分钟后显著降低血糖峰值水平。在四氧嘧啶诱导的糖尿病大鼠中，每日口服安博因香茶菜乙醇提取物可显著降低血糖。此外，给予安博因香茶菜乙醇提取物15天可显著降低血清总胆固醇、甘油三酯含量，而高密度脂蛋白胆固醇、总蛋白和钙则有效增加。此外，安博因香茶菜乙醇提取物的作用表现为血清淀粉酶显著升高和血清脂肪酶降低。组织学检查显示，安博因香茶菜乙醇提取物使糖尿病大鼠受损的胰腺结构几乎恢复正常。研究清楚地表明，安博因香茶菜叶乙醇提取物具有通过恢复胰腺组织功能和促胰岛素分泌作用介导的降血糖和降血脂作用。
