Abdel Rahman Z A, Fadali G A, Abou Basha L M
Department of Immunology, Alexandria University.
J Egypt Soc Parasitol. 1990 Dec;20(2):521-8.
Liver biopsies from 5 patients with established fascioliasis, before and after bithionol treatment were studied by immunoalkaline phosphatase technique for relative distribution of T cells and their subpopulations. T cell and its subsets are defined for OKT3+ (pan T), OKT4+ (helper/inducer) and OKT8+ (suppressor/cytotoxic) cells by using mouse monoclonal antibodies. Before bithionol treatment, lymphocytic infiltration in all hepatic lesions were predominantly of OKT3+ (pan T) lymphocytes. The distribution of OKT8+ cells was moderate to severe in comparison to the few OKT4+ cells presentation. After bithionol a noticeable regression of the OKT3 lymphocytic in all liver sections. The majority of the lymphocytic infiltration was of the OKT8+ cells, in comparison to the absence of the OKT4+ ones. This may indicate that suppressor/cytotoxic lymphocytes may have a role in the immune regulation of the disease and the mode of action of bithionol is by the accentuation of this immunoregulatory effect.
采用免疫碱性磷酸酶技术，对5例已确诊的姜片虫病患者在硫双二氯酚治疗前后的肝活检组织进行研究，以观察T细胞及其亚群的相对分布情况。通过使用小鼠单克隆抗体，将T细胞及其亚群定义为OKT3 +（全T细胞）、OKT4 +（辅助/诱导性T细胞）和OKT8 +（抑制/细胞毒性T细胞）。在硫双二氯酚治疗前，所有肝脏病变中的淋巴细胞浸润主要为OKT3 +（全T细胞）淋巴细胞。与少量的OKT4 +细胞相比，OKT8 +细胞的分布为中度至重度。硫双二氯酚治疗后，所有肝切片中OKT3淋巴细胞均有明显消退。与OKT4 +细胞缺失相比，大多数淋巴细胞浸润为OKT8 +细胞。这可能表明抑制/细胞毒性淋巴细胞可能在该疾病的免疫调节中起作用，硫双二氯酚的作用方式是增强这种免疫调节作用。
