Pallet Nicolas, Thervet Eric, Desjardins Michel
Département de Microbiologie et Immunologie Montréal, QC, Canada.
Front Genet. 2011 Dec 28;2:98. doi: 10.3389/fgene.2011.00098. eCollection 2011.
Oligonucleotide microarray technology has created a small revolution in the transplant community because it has helped to decipher previously unknown molecular processes involved in allograft pathology, redefined molecular patterns of diseases that are indistinguishable at the pathological level and made possible the definition of new prognostic factors for long-term graft outcomes. However, given the tremendous complexity of the biological processes that are involved in the pathology of a transplanted organ, the interpretation of transcriptomic data can be speculative and oversimplified. Here, we discuss critical considerations regarding the nature of the object studied by cDNA microarray technology, the means by which it is observed, the interpretation of the observations, and whether the observations make sense in the context of transplant-related scientific questions. Given these limitations, we believe that global approaches based on more functional biological intermediates are necessary for a better understanding of the molecular processes that regulate the physiopathology of the transplanted organ.
寡核苷酸微阵列技术在移植领域引发了一场小革命，因为它有助于破解同种异体移植病理学中以前未知的分子过程，重新定义在病理水平上难以区分的疾病分子模式，并使得定义长期移植结果的新预后因素成为可能。然而，鉴于移植器官病理学所涉及的生物过程极其复杂，转录组数据的解读可能具有推测性且过于简化。在此，我们讨论关于cDNA微阵列技术所研究对象的性质、观察手段、观察结果的解读以及这些观察结果在移植相关科学问题背景下是否合理等关键问题。鉴于这些局限性，我们认为基于更多功能性生物中间体的全局方法对于更好地理解调节移植器官生理病理学的分子过程是必要的。
