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[干旱胁迫及复水对喀斯特地区柏木幼苗活性氧清除系统的影响]

[Effects of drought stress and re-watering on the active oxygen scavenging system of Cupressus funebris seedlings in Karst area].

作者信息

Liu Jin-Chun, Zhong Zhang-Cheng, He Yue-Jun

机构信息

Ministry of Education Key Laboratory of Eco-environment in Three Gorges Reservoir Region, Southwest China University, Chongqing 400715, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2011 Nov;22(11):2836-40.

Abstract

This paper studied the active oxygen scavenging system of Cupressus funebris seedlings under drought condition and the recovery capability of the system after re-watering, aimed to understand the adaptation mechanisms of C. funebris to the 'drought and re-watering' environment in Karst area. With the increasing time of drought stress, the seedling's relative water content (RWC) decreased, soluble protein concentration increased first and decreased then, and malondialdehyde (MDA) content increased consistently. The MDA content recovered to the level of the control (CK) when re-watering was implemented within 2 weeks of drought, but could not when the re-watering was made after 4 and 6 weeks of drought. Under drought stress, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) increased consistently. After rewatering, the SOD activity had somewhat decrease but still remained at a higher level than the CK, and the POD and CAT activities decreased to the CK level when suffered mild stress but had less decrement when suffered severe stress. It was concluded that C. funebris seedlings could resist mild drought stress via increasing their soluble protein concentration and inhibiting membrane lipid peroxidation, but could not resist severe drought stress because of the irreversible damage of their membrane structure.

摘要

本文研究了干旱条件下柏木幼苗的活性氧清除系统及其复水后的恢复能力,旨在了解柏木对喀斯特地区“干旱-复水”环境的适应机制。随着干旱胁迫时间的增加,幼苗的相对含水量(RWC)降低,可溶性蛋白浓度先升高后降低,丙二醛(MDA)含量持续增加。在干旱2周内进行复水时,MDA含量恢复到对照(CK)水平,但在干旱4周和6周后进行复水时则不能恢复。干旱胁迫下,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性持续增加。复水后,SOD活性有所下降,但仍高于CK水平,POD和CAT活性在轻度胁迫时下降至CK水平,在重度胁迫时下降幅度较小。研究得出结论,柏木幼苗可通过增加可溶性蛋白浓度和抑制膜脂过氧化来抵抗轻度干旱胁迫,但由于其膜结构的不可逆损伤而不能抵抗重度干旱胁迫。

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