Wang Cong, Zhu Yue-Lin, Yang Li-Fei, Yang Heng-Shan
College of Agriculture, Inner Mongolia University for Nationalities, Tongliao 028042, Inner Mongolia, China.
Ying Yong Sheng Tai Xue Bao. 2011 Nov;22(11):2883-93.
By adopting vermiculite culture, this paper studied the malondialdehyde (MDA) content and polyamines (PAs) metabolism in the seeds of two vegetable soybean cultivars with different salt tolerance under the stress of 100 mmol NaCl x L(-1). NaCl stress increased the MDA contents in the seeds of the two cultivars significantly, but the increment was lower for salt-resistant cultivar 'Lülingtezao' (LL) than for salt-sensitive cultivar 'Lixianggaochan 95-1' (LX). In the whole period of NaCl stress, LL seeds had higher contents of free spermine (Spm), conjugated Spm, conjugated spermidine (Spd), bound Spd and bound putrescine (Put), higher values of (Spd+Spm)/Put and (cPAs+bPAs)/fPAs, and lower value of Put/PAs, and had higher content of free Spd in the mid and late period (9-15 d) of NaCl stress, as compared with LX seeds. In a longer term (6-15 d) of the stress, LL seeds maintained a higher activity of arginine decarboxylase (ADC) and a lower activity of polyamine oxidase (PAO). All the results suggested that LL seeds had stronger capabilities for polyamines synthesis and for the transformation of Put to Spd and Spm and of free polyamine to conjugated and bound polyamines, which effectively inhibited the membrane lipid peroxidation, possibly being one of the important reasons for the stronger salt tolerance of LL.
采用蛭石培养法，研究了100 mmol NaCl·L⁻¹胁迫下两个耐盐性不同的菜用大豆品种种子中丙二醛（MDA）含量和多胺（PAs）代谢情况。NaCl胁迫显著增加了两个品种种子中的MDA含量，但耐盐品种‘绿领特早’（LL）的增幅低于盐敏感品种‘理想高产95 - 1’（LX）。在NaCl胁迫的整个时期，与LX种子相比，LL种子具有较高的游离精胺（Spm）、结合态Spm、结合态亚精胺（Spd）、束缚态Spd和束缚态腐胺（Put）含量，较高的（Spd + Spm）/Put和（cPAs + bPAs）/fPAs值，以及较低的Put/PAs值，并且在NaCl胁迫的中期和后期（9 - 15 d）具有较高的游离Spd含量。在较长时间（6 - 15 d）的胁迫下，LL种子保持较高的精氨酸脱羧酶（ADC）活性和较低的多胺氧化酶（PAO）活性。所有结果表明，LL种子具有更强的多胺合成能力，以及将Put转化为Spd和Spm、将游离多胺转化为结合态和束缚态多胺的能力，这有效地抑制了膜脂过氧化，可能是LL耐盐性较强的重要原因之一。
