基于适体的病毒生存力阻抗传感器。

Aptamer-based viability impedimetric sensor for viruses.

机构信息

Department of Chemistry, University of Ottawa, 10 Marie Curie, Ottawa, Ontario K1N 6N5, Canada.

出版信息

Anal Chem. 2012 Feb 21;84(4):1813-6. doi: 10.1021/ac203412m. Epub 2012 Feb 1.

DOI:10.1021/ac203412m

Abstract

The development of aptamer-based viability impedimetric sensor for viruses (AptaVISens-V) is presented. Highly specific DNA aptamers to intact vaccinia virus were selected using cell-SELEX technique and integrated into impedimetric sensors via self-assembly onto a gold microelectrode. Remarkably, this aptasensor is highly selective and can successfully detect viable vaccinia virus particles (down to 60 virions in a microliter) and distinguish them from nonviable viruses in a label-free electrochemical assay format. It also opens a new venue for the development of a variety of viability sensors for detection of many microorganisms and spores.

摘要

本文介绍了基于适体的病毒生存力阻抗传感器（AptaVISens-V）的开发。使用细胞 SELEX 技术筛选出对完整牛痘病毒具有高特异性的 DNA 适体，并通过自组装到金微电极上整合到阻抗传感器中。值得注意的是，这种适体传感器具有高度选择性，能够成功检测到有活力的牛痘病毒颗粒（低至 60 个病毒颗粒在微升），并在无标记的电化学分析格式中区分它们与无活力的病毒。它还为开发用于检测许多微生物和孢子的各种生存力传感器开辟了新途径。