Sudhamani Chittanahalli N, Bhojya Naik Halehatty S, Girija D
Department of Industrial Chemistry, School of Chemical Sciences, Kuvempu University, Shankaraghatta, Karnataka, India.
Nucleosides Nucleotides Nucleic Acids. 2012;31(2):130-46. doi: 10.1080/15257770.2011.644369.
Four new Co(III) complexes, namely Co(cq)(3)(3), Co(phen)(2)(cq)(3), [Co(bnp)(3)] (PF(6))(3), and Co(phen)(2)(bnp)(3) (where cq = chromeno[2,3-b]quinoline, phen = 1,10-phenanthroline and bnp = dibenzo[b,g][1,8]naphthyridine), were synthesized and structurally characterized. Spectroscopic data suggested an octahedral geometry for all the complexes. Binding studies of these complexes with double-stranded (ds)DNA were analyzed by absorption spectra, viscosity, and thermal denaturation studies. The results revealed that the metal complex intercalates into the DNA base stack as intercalator. The oxidative cleavage activities of the complexes were studied with supercoiled pUC19 DNA using gel electrophoresis and the results show that the complexes have potent nuclease activity.
合成并表征了四种新型钴（III）配合物，即Co(cq)(3)(3)、Co(phen)(2)(cq)(3)、[Co(bnp)(3)] (PF(6))(3)和Co(phen)(2)(bnp)(3)（其中cq = 色烯并[2,3 - b]喹啉，phen = 1,10 - 菲咯啉，bnp = 二苯并[b,g][1,8]萘啶）。光谱数据表明所有配合物均为八面体几何构型。通过吸收光谱、粘度和热变性研究分析了这些配合物与双链（ds）DNA的结合研究。结果表明金属配合物作为嵌入剂嵌入DNA碱基堆积中。使用凝胶电泳研究了配合物对超螺旋pUC19 DNA的氧化切割活性，结果表明配合物具有强大的核酸酶活性。
