Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
J Microencapsul. 2012;29(5):429-36. doi: 10.3109/02652048.2012.655332. Epub 2012 Feb 6.
Harsh gastric condition causes low bioavailability of probiotics when supplied orally. Polymeric encapsulation has successfully protected bacteria from harsh gastric condition and ultimately increased persistency and multiplication at the targeted region. In this study, we encapsulated LS29 into ACA microcapsules and characterized them. The survivability and release of LS29 from LS29-loaded ACA microcapsules in SGF and SIF were studied. Encapsulation efficiency of LS29 in ACA microcapsules was 99.9%. Approximately 70% of bacteria survived at pH 2 by 120 min after encapsulation. Although not much difference of the survivability of LS29 encapsulated into ACA and FDACA was observed, freeze-drying improved the controlled-release of LS29 in SIF and also showed better storage survivability at 4°C for 8 weeks. Furthermore, investigation of in vitro production of cytokines in RAW264.7 showed high level of induction of TNF-α and IL-10. These in vitro results support that the LS29 might have a balanced immunomodulatory effect.
当通过口服提供时，恶劣的胃部环境会导致益生菌的生物利用度降低。聚合包封成功地保护了细菌免受恶劣的胃部环境的影响，最终增加了在目标区域的持久性和繁殖能力。在这项研究中，我们将 LS29 封装到 ACA 微胶囊中并对其进行了表征。研究了 LS29 负载的 ACA 微胶囊在 SGF 和 SIF 中的生存能力和释放。LS29 在 ACA 微胶囊中的包封效率为 99.9%。封装后 pH 2 下经过 120 分钟，约有 70%的细菌存活。尽管观察到 LS29 封装到 ACA 和 FDACA 中的存活率没有太大差异，但冷冻干燥改善了 LS29 在 SIF 中的控制释放，并且在 4°C 下 8 周的储存存活率也更好。此外，在 RAW264.7 中进行的细胞因子体外生产研究表明，TNF-α和 IL-10 的诱导水平很高。这些体外结果表明，LS29 可能具有平衡的免疫调节作用。
