College of Life Sciences, Northeast Forestry University, Harbin, P.R. China.
J Microencapsul. 2013;30(6):511-8. doi: 10.3109/02652048.2012.758178. Epub 2013 Feb 14.
Six kinds of Bifidobacterium bifidum microcapsules were prepared by extrusion methods, emulsion methods and coacervation methods. Effects of preparation methods on the survival of encapsulated B. bifidum were examined. Results showed that microcapsules prepared by emulsion method with alginate and chitosan exhibited the best protection for B. bifidum. The diameter was 10-20 µm, encapsulation efficiency was 90.36% and the live cell amount was 3.01 × 10₉ cfu/g after freeze-drying. Encapsulated cells exhibited significantly higher resistance to artificial gastrointestinal juice and the cell numbers were above 10₉ cfu/g after exposure to simulated gastric (pH 1.2) and bile salt (1%, w/v). Cell numbers of microencapsulated B. bifidum was 8.61 × 10₈ cfu/g after storage at 37°C, relative humidity 60%-65% for 3 months. Results indicated microcapsules prepared with alginate and chitosan by emulsion method could successfully protect B. bifidum against adverse conditions and it might be useful in the delivery of probiotic cultures as a functional food.
采用挤压法、乳化法和共凝聚法制备了 6 种双歧杆菌微胶囊。考察了制备方法对包埋双歧杆菌存活率的影响。结果表明，用海藻酸钠和壳聚糖制备的乳化法微胶囊对双歧杆菌的保护效果最好。微胶囊的直径为 10-20 µm，包埋效率为 90.36%，冷冻干燥后活菌数为 3.01×109 cfu/g。包埋细胞对人工胃肠液具有显著的抵抗力，在模拟胃液（pH 1.2）和胆盐（1%，w/v）暴露后，细胞数仍保持在 109 cfu/g 以上。在 37°C、相对湿度 60%-65%下贮藏 3 个月后，微囊化双歧杆菌的活菌数为 8.61×108 cfu/g。结果表明，用海藻酸钠和壳聚糖通过乳化法制备的微胶囊可以成功地保护双歧杆菌免受不利条件的影响，这可能有助于将益生菌培养物作为功能性食品进行传递。
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