Hattori T
Department of Orthopedic Surgery, Nagoya University School of Medicine, Aichi, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1990 Sep;64(9):824-34.
The influence of fibrin glue on ectopic osteo-chondrogenesis induced by bone morphogenetic protein (BMP), which was extracted from decalcified rabbit long bones, was investigated histologically, radiographically, and electronmicroscopically. The mixtures, consisting of fibrin glue and BMP, were implanted between femoral biceps muscles of AKR mice. They were examined 3 days, 5 days, 1 week, and 2 weeks after implantation, respectively. The fibrin glue did not affect osteo-chondrogenesis by BMP under the mixture conditions, and new bone formation was seen almost in every implant. The fine network of fibrin glue seemed to be effective for adhesion, differentiation of BMP responding cells and was well cooperated on BMP. Fibrin glue network could control the area of osteo-chondrogenesis by BMP. It might be due to restriction of diffusion of BMP. In addition, the whole shape of the newly formed cartilage and bone was influenced by the quantity of Aprotinin administered to fibrin glue. Fibrin glue may be regarded as an effective cooperator of BMP in the case of clinical application of BMP in the future.
研究了从脱钙兔长骨中提取的纤维蛋白胶对骨形态发生蛋白（BMP）诱导的异位骨软骨生成的影响，采用组织学、放射学和电子显微镜检查方法。将由纤维蛋白胶和BMP组成的混合物植入AKR小鼠股二头肌之间。分别在植入后3天、5天、1周和2周进行检查。在混合条件下，纤维蛋白胶不影响BMP诱导的骨软骨生成，几乎在每个植入物中都可见新骨形成。纤维蛋白胶的精细网络似乎对BMP反应细胞的黏附、分化有效，并与BMP良好协作。纤维蛋白胶网络可以控制BMP诱导的骨软骨生成区域。这可能是由于BMP扩散受限所致。此外，给予纤维蛋白胶的抑肽酶数量会影响新形成的软骨和骨的整体形态。在未来BMP临床应用中，纤维蛋白胶可被视为BMP的有效协同剂。
