Agriculture College, Hainan University, Haikou, China.
Asian Pac J Trop Med. 2012 Mar;5(3):169-74. doi: 10.1016/S1995-7645(12)60019-4.
To investigate whether cytochalasin D can induce antitumor activities in a tumor model.
Murine CT26 colorectal carcinoma cells were cultured in vitro and cytochalasin D was used as a cytotoxic agent to detect its capabilities of inhibiting CT26 cell proliferation and inducing cell apoptosis by MTT and a TUNEL-based apoptosis assay. Murine CT26 tumor model was established to observe the tumor growth and survival time. Tumor tissues were used to detect the microvessel density by immunohistochemistry. In addition, alginate encapsulated tumor cell assay was used to quantify the tumor angiogenesis in vivo.
Cytochalasin D inhibited CT26 tumor cell proliferation in time and dose dependent manner and induced significant CT26 cell apoptosis, which almost reached the level induced by the positive control nuclease. The optimum effective dose of cytochalasin D for in vivo therapy was about 50 mg/kg. Cytochalasin D in vivo treatment significantly inhibited tumor growth and prolonged the survival times in CT26 tumor-bearing mice. The results of immunohistochemistry analysis and alginate encapsulation assay indicated that the cytochalasin D could effectively inhibited tumor angiogenesis.
Cytochalasin D inhibits CT26 tumor growth potentially through inhibition of cell proliferation, induction of cell apoptosis and suppression of tumor angiogenesis.
研究细胞松弛素 D 是否能在肿瘤模型中诱导抗肿瘤活性。
体外培养小鼠 CT26 结直肠癌细胞，用细胞松弛素 D 作为细胞毒性剂，通过 MTT 和 TUNEL 法检测其抑制 CT26 细胞增殖和诱导细胞凋亡的能力。建立小鼠 CT26 肿瘤模型，观察肿瘤生长和生存时间。用免疫组织化学法检测肿瘤组织微血管密度。此外，还采用藻酸盐包埋肿瘤细胞实验法检测体内肿瘤血管生成。
细胞松弛素 D 呈时间和剂量依赖性抑制 CT26 肿瘤细胞增殖，并诱导显著的 CT26 细胞凋亡，其诱导凋亡的效果几乎与阳性对照核酸酶相当。体内治疗的最佳有效剂量约为 50mg/kg。细胞松弛素 D 体内治疗明显抑制肿瘤生长，延长 CT26 荷瘤小鼠的生存时间。免疫组织化学分析和藻酸盐包埋实验结果表明，细胞松弛素 D 能有效抑制肿瘤血管生成。
细胞松弛素 D 通过抑制细胞增殖、诱导细胞凋亡和抑制肿瘤血管生成，可能抑制 CT26 肿瘤生长。
