Department of Quality Assurance & Natural Products, PDM College of Pharmacy, Bahadurgarh, India.
Asian Pac J Trop Med. 2012 Mar;5(3):225-30. doi: 10.1016/S1995-7645(12)60029-7.
To study the isolation and characterization of the constituent responsible for the cytotoxic activity of the ethanolic extract of stem of Capparis decidua (C. decidua).
The preliminary cytotoxic effect of isolated compound (β-Sitosterol triacontenate) was investigated by MTT assay on A549 solid tumor cells.
IC(50) value of the β-Sitosterol triacontenate was found to be 1 μM. The cytotoxic activity increased in a dose dependent manner in case of β-Sitosterol triacontenate.
The data therefore provide direct evidence for the role of β-Sitosterol triacontenate as a potent antimetastatic agent, which can markedly inhibit the metastatic and invasive capacity of malignant cells.
研究负责山木鳖茎乙醇提取物细胞毒性的成分的分离和鉴定。
通过 MTT 法测定分离得到的化合物（β-谷甾醇三酸酯）对 A549 实体瘤细胞的初步细胞毒性作用。
β-谷甾醇三酸酯的 IC50 值为 1 μM。β-谷甾醇三酸酯的细胞毒性活性呈剂量依赖性增加。
因此，数据为β-谷甾醇三酸酯作为一种有效的抗转移剂提供了直接证据，它可以显著抑制恶性细胞的转移和侵袭能力。
