Department of Chemistry, University at Albany, SUNY, Albany, NY 12222, United States.
Anal Chim Acta. 2012 Mar 9;718:78-83. doi: 10.1016/j.aca.2011.12.059. Epub 2012 Jan 2.
This proof-of-concept study demonstrated the potential of Raman microspectroscopy for nondestructive identification of traces of sweat for forensic purposes. Advanced statistical analysis of Raman spectra revealed that dry sweat was intrinsically heterogeneous, and its biochemical composition varies significantly with the donor. As a result, no single Raman spectrum could adequately represent sweat traces. Instead, a multidimensional spectroscopic signature of sweat was built that allowed for the presentation of any single experimental spectrum as a linear combination of two fluorescent backgrounds and three Raman spectral components dominated by the contribution from lactate, lactic acid, urea and single amino acids.
本概念验证研究表明，拉曼显微镜用于非破坏性鉴定用于法医目的的汗迹具有潜力。对拉曼光谱的高级统计分析表明，干燥的汗液本质上是不均匀的，其生化组成随供体有很大差异。因此，没有单个拉曼光谱可以充分代表汗迹。相反，建立了汗液的多维光谱特征，使得任何单个实验光谱都可以表示为两个荧光背景和三个拉曼光谱分量的线性组合，这三个拉曼光谱分量主要由乳酸、乳酸、尿素和单个氨基酸的贡献主导。
