College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Gene. 2012 Apr 15;497(2):336-9. doi: 10.1016/j.gene.2012.01.035. Epub 2012 Jan 28.
Escherichia coli (E. coli) that produces adhesin F18 is the main pathogen responsible for porcine post-weaning diarrhea and edema disease. The receptor for E. coli F18 has not been described in pigs, however the alpha (1,2)-fucosyltransferase (FUT1) gene on chromosome 6 has been proposed as a candidate. The objective of this study, therefore, was to investigate the relationship between FUT1 gene expression and E. coli F18 receptor in Sutai pigs of different ages (8-, 18-, 30- and 35-day-old). FUT1 gene expression was detected in 11 pig tissues with the highest level in lung, and expressed consistently at the four time points. In most tissues, FUT1 gene expression levels decreased from days 8 to 18, then continually increased on days 30 and 35, with expression around weaning time higher than that on day 8. Gene ontology and pathway analysis showed that FUT1 was involved in 32 biological processes, mainly those integral to the membrane, or involved in glycosylation, as well as regulation of binding, interestingly participating in three pathways related to glycosphingolipid biosynthesis. From this analysis and the high linkage disequilibrium between the FUT1 gene and the E. coli F18 receptor locus, we can speculate that higher expression of the FUT1 gene in small intestine is beneficial to the formation of receptors to the E. coli F18 strain and is related to the sensitivity to the pathogen.
产黏附素 F18 的大肠杆菌（E. coli）是引起仔猪断奶后腹泻和水肿病的主要病原体。然而，尚未在猪中描述大肠杆菌 F18 的受体，但是已经提出 6 号染色体上的α（1,2）-岩藻糖基转移酶（FUT1）基因作为候选物。因此，本研究的目的是研究不同年龄（8、18、30 和 35 日龄）苏太猪中 FUT1 基因表达与大肠杆菌 F18 受体之间的关系。在 11 种猪组织中检测到 FUT1 基因的最高表达水平在肺中，并且在四个时间点均一致表达。在大多数组织中，FUT1 基因表达水平从第 8 天到第 18 天下降，然后在第 30 天和第 35 天持续增加，断奶时的表达水平高于第 8 天。基因本体论和途径分析表明，FUT1 参与了 32 个生物学过程，主要涉及膜内过程，或参与糖基化以及结合调节，有趣的是参与了三个与糖鞘脂生物合成相关的途径。从这种分析以及 FUT1 基因与大肠杆菌 F18 受体基因座之间的高度连锁不平衡，我们可以推测小肠中 FUT1 基因的高表达有利于形成对大肠杆菌 F18 株的受体，并且与对病原体的敏感性有关。
