Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ Buenos Aires, Argentina.
J Virol Methods. 2012 Apr;181(1):80-5. doi: 10.1016/j.jviromet.2012.01.016. Epub 2012 Jan 27.
The aim of this work was to study the in vitro replication of bovine herpesvirus types 1 and 5 (BoHV-1 and 5) at the beginning and end of the logarithmic growth phase of Madin-Darby Bovine Kidney (MDBK) cells. The replication kinetics and size of lysis and infection plaques of the field isolates 09/210 (BoHV-1) and 97/613 (BoHV-5) and the reference strains BoHV-1.1 Los Angeles 38 (LA38), BoHV-1.1 Cooper, BoHV-5a N569 and BoHV-5b A663 were evaluated. The highest mean virus titre was recorded for N569, followed by LA38 and 97/613. For most of the viruses, the virus titre values increased from 24 h post-infection (hpi) up to 48 hpi and then, they remained unchanged up to 72 hpi. However, the virus titre for 09/210 was significantly lower and a slight, steady increase was observed from 24 to 72 hpi. Furthermore, the largest lysis and infection plaques were recorded for 97/613 and LA38, respectively. According to this work, it is evident that there is a relationship between the replication of BoHV and the multiplication stage of MDBK cells. The results of this study contribute to the understanding of the replication behaviour in cell cultures of several strains of BoHV, which is critical for the rational design of in vitro experiments and vaccine production.
本工作旨在研究牛疱疹病毒 1 型和 5 型（BoHV-1 和 BoHV-5）在马迪-达比牛肾（MDBK）细胞对数生长期开始和结束时的体外复制。评估了 09/210（BoHV-1）和 97/613（BoHV-5）田间分离株以及参考株 BoHV-1.1 Los Angeles 38（LA38）、BoHV-1.1 Cooper、BoHV-5a N569 和 BoHV-5b A663 的复制动力学和裂解及感染斑大小。N569 的平均病毒滴度最高，其次是 LA38 和 97/613。对于大多数病毒，病毒滴度值从感染后 24 小时（hpi）增加到 48 hpi，然后在 72 hpi 之前保持不变。然而，09/210 的病毒滴度显著较低，从 24 到 72 hpi 观察到轻微、稳定的增加。此外，最大的裂解和感染斑分别记录为 97/613 和 LA38。根据这项工作，很明显 BoHV 的复制与 MDBK 细胞的增殖阶段之间存在关系。本研究的结果有助于了解几种 BoHV 株在细胞培养中的复制行为，这对于合理设计体外实验和疫苗生产至关重要。
