Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan.
Regul Toxicol Pharmacol. 2012 Apr;62(3):419-24. doi: 10.1016/j.yrtph.2012.01.003. Epub 2012 Jan 28.
The genotoxicity of fullerene C(60) nanoparticles was evaluated in vivo with comet assays using the lung cells of rats given C(60) nanoparticles. The C(60) nanoparticles were intratracheally instilled as a single dose at 0.5 or 2.5mg/kg or repeated dose at 0.1 or 0.5mg/kg, once a week for 5 weeks, to male rats. The lungs were obtained 3 or 24h after a single instillation and 3h after repeated instillation. Inflammatory responses were observed in the lungs obtained 24h after a single instillation at 2.5mg/kg and repeated instillation at 0.5mg/kg. Histopathological examinations revealed that C(60) nanoparticles caused slight changes including hemorrhages in alveoli and the cellular infiltration of macrophages and neutrophils in alveoli. In comet assays using rat lung cells, no increase in % Tail DNA was found in any group given C(60) nanoparticles. These findings indicate that C(60) nanoparticles had no potential for DNA damage in comet assays using the lungs cells of rats given C(60) even at doses causing inflammation.
采用彗星试验，以给予 C（60）纳米粒子的大鼠的肺细胞为研究对象，对富勒烯 C（60）纳米粒子的遗传毒性进行了体内评估。将 C（60）纳米粒子以 0.5 或 2.5mg/kg 的单剂量经气管内滴注，或以 0.1 或 0.5mg/kg 的重复剂量，每周一次，共 5 周，给予雄性大鼠。在单次滴注后 3 或 24 小时以及重复滴注后 3 小时获取肺部组织。在 2.5mg/kg 单次滴注和 0.5mg/kg 重复滴注后 24 小时获取的肺部组织中观察到炎症反应。组织病理学检查显示，C（60）纳米粒子导致轻微变化，包括肺泡出血和肺泡中巨噬细胞和中性粒细胞的细胞浸润。在使用大鼠肺细胞的彗星试验中，未发现给予 C（60）纳米粒子的任何组的%尾 DNA 增加。这些发现表明，即使在引起炎症的剂量下，给予 C（60）纳米粒子的大鼠肺细胞的彗星试验中，C（60）纳米粒子没有潜在的 DNA 损伤。
