Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki, 305-8569, Japan.
J Appl Toxicol. 2013 Oct;33(10):1053-60. doi: 10.1002/jat.2810. Epub 2012 Aug 31.
The genotoxicity of multi-walled carbon nanotubes (MWCNTs) was evaluated in vivo with comet assays using the lung cells of rats given MWCNTs. The MWCNTs were intratracheally instilled as a single dose at 0.2 or 1.0 mg kg(-1) or a repeated dose at 0.04 or 0.2 mg kg(-1) , once a week for 5 weeks, to male rats. The rats were sacrificed 3 or 24 h after the single instillation and were sacrificed 3 h after the last instillation in the repeated instillation groups. Histopathological examinations of the lungs revealed that MWCNTs caused inflammatory changes including the infiltration of macrophages and neutrophils after a single instillation and repeated instillation at both doses. In comet assays using rat lung cells, no changes in % Tail DNA were found in any group given MWCNTs. These findings indicate that MWCNTs do not have the potential to cause DNA damage in comet assays using the lung cells of rats given MWCNTs at doses causing inflammatory responses.
采用彗星试验，通过气管内滴注方式将多壁碳纳米管（MWCNTs）给予大鼠，在体内评估其遗传毒性。MWCNTs 以 0.2 或 1.0mg/kg 单次剂量，或 0.04 或 0.2mg/kg 每周一次重复剂量，给予雄性大鼠。单次滴注后 3 或 24 小时处死大鼠，重复滴注组最后一次滴注后 3 小时处死。肺组织病理学检查显示，MWCNTs 在单次和重复滴注两种剂量下均引起炎症改变，包括巨噬细胞和中性粒细胞浸润。用大鼠肺细胞进行彗星试验，未发现给予 MWCNTs 的任何组的%尾 DNA 发生变化。这些发现表明，在引起炎症反应的剂量下，MWCNTs 不具有在给予 MWCNTs 的大鼠肺细胞彗星试验中引起 DNA 损伤的潜力。
