Wood W G
Klinische Laboratorien, Medizinische Universität zu Lübeck.
J Clin Chem Clin Biochem. 1990 Jul;28(7):481-3.
This short communication describes the use of spiroadamantane-1,2 dioxetane substrates for alkaline phosphatase and beta-D-galactosidase in the development of luminescence-enhanced enzyme immunoassays. Until the present work, only peroxidase-luminol/peroxidase systems had been used in such assays. The light reaction kinetics were studied from the time of initiation of the light reaction up to 2 days later. The methods is illustrated by the determination of myeloperoxidase, based on microtitre plate technology, coupled with a microtitre plate luminometer which needs no injector. The alkaline phosphatase and peroxidase systems were compared.
本短文描述了在发光增强酶免疫分析的开发中，螺金刚烷-1,2-二氧杂环丁烷底物用于碱性磷酸酶和β-D-半乳糖苷酶的情况。在本研究之前，此类分析仅使用过过氧化物酶-鲁米诺/过氧化物酶体系。研究了从光反应开始直至两天后的光反应动力学。以基于微量滴定板技术并结合无需注射器的微量滴定板发光计来测定髓过氧化物酶为例，对该方法进行了说明。并对碱性磷酸酶和过氧化物酶体系进行了比较。
