Wood W G, Hantke U, Gross A J
J Clin Chem Clin Biochem. 1985 Jan;23(1):47-9.
We describe the development of a single-reagent addition to initiate the light output in the oxidation of 6-(N-(4-aminobutyl-N-ethyl)isoluminol) hemisuccinamide (ABEI-H) as the final step in solid-phase luminescence immunoassays. Up until now, the disadvantage of luminol-based luminescence was the need to inject three solutions to optimise stability and performance in immunoassays. By combining these reagents, (pseudoperoxidase, sodium hydroxide and hydrogen peroxide), it has become possible to develop a system which is stable over several hours, and which, almost as a "spin-off" product, has a light-output between 2 and 5-fold that of the original system at the same time hardly altering the reagent blank. Such a system has the advantage that it can be easily adapted to automatic closed systems when these become available for luminescence immunoassays. With this implementation, single-injection light-initiation reactions are not restricted to acridinium-ester or fluorescein isothiocyanate luminescence.
我们描述了一种单一试剂添加物的研发情况，该添加物用于引发6-(N-(4-氨基丁基-N-乙基)异鲁米诺)半琥珀酰胺(ABEI-H)氧化过程中的光输出，这是固相发光免疫分析的最后一步。到目前为止，基于鲁米诺的发光的缺点是在免疫分析中需要注入三种溶液以优化稳定性和性能。通过将这些试剂（假过氧化物酶、氢氧化钠和过氧化氢）组合，已能够开发出一种在数小时内稳定的系统，并且几乎作为一种“副产品”，该系统的光输出是原始系统的2至5倍，同时几乎不改变试剂空白。这样的系统具有如下优点：当发光免疫分析可用自动封闭系统时，它可以很容易地适应这些系统。通过这种实施方式，单注射光引发反应并不局限于吖啶酯或异硫氰酸荧光素发光。
