Braun J P, Ouedraogo G, Thorel B, Médaille C, Rico A G
Laboratoire Associé INRA Toxicologie Biochimique et Métabolique, Ecole Nationale Vétérinaire, Toulouse, France.
J Clin Chem Clin Biochem. 1990 Jul;28(7):493-5.
Commercially available chromogenic substrates for the assay of alpha-amylase were tested for specificity in dog plasma. Blocked alpha-4-nitrophenylmaltoheptaoside and non-blocked beta-4-nitrophenylmaltoheptaoside showed no interference with glucoamylase and were suitable for the measurement of alpha-amylase in dog plasma. In contrast, an alpha-4-nitrophenylmaltoheptaoside showed interference, and was therefore an inappropriate substrate. Reference values with the blocked substrate in a group of 82 non-selected 3 month- to 10 year-old male and female dogs were 355 +/- 131 U/l (mean +/- standard deviation) at 30 degrees C.
对市售的用于检测α-淀粉酶的显色底物在犬血浆中的特异性进行了测试。封闭的α-4-硝基苯麦芽庚糖苷和未封闭的β-4-硝基苯麦芽庚糖苷对葡糖淀粉酶无干扰，适用于测定犬血浆中的α-淀粉酶。相比之下，一种α-4-硝基苯麦芽庚糖苷显示出干扰，因此是不合适的底物。在一组82只未经过挑选的3个月至10岁的雄性和雌性犬中，在30℃下使用封闭底物的参考值为355±131 U/l（平均值±标准差）。
