Hohenwallner W, Wimmer E, Sommer R
J Clin Chem Clin Biochem. 1979 Jun;17(6):349-52.
The enzymatic method of H. W. Schiwara (1972) Z. Klim. Chem. Klin. Biochem. 10,12--16 (reagents by Smith Kline Instruments), using the enzymatic reaction sequence alpha-amylase -- alpha-glucosidase -- hexokinase/glucose 6-phosphate dehydrogenase for the determination of alpha-amylase was evaluated on the ABA-100. The coefficient of variation for control sera and human pooled serum was 0.9--4.2% within series, and 1.4--6.6% day to day. Reference values for a healthy population (212 blood donors) in sera were 13--79 U/1 (+/- 2 SD), mean 46 U/1. In catch urines the values did not show a normal distribution; the minimal and maximal range for men was 58--385 U/1, for women 7--318 U/1. The kinetic curve of the enzymatic test was measured and the influence of glucose and linearity studied. In comparison with the enzymatic test, the chromogenic method Amlyochrom Roche was tested on the sera and urine of patients. The coefficient of correlation in sera was r = 0.975, in urine r = 0.965.
采用H. W. Schiwara（1972年，《临床化学与临床生物化学杂志》第10卷，第12 - 16页，试剂由史克公司提供）的酶法，利用α -淀粉酶 - α -葡萄糖苷酶 - 己糖激酶/葡萄糖6 -磷酸脱氢酶的酶促反应序列来测定α -淀粉酶，并在ABA - 100上进行了评估。对照血清和人混合血清的批内变异系数为0.9 - 4.2%，批间变异系数为1.4 - 6.6%。健康人群（212名献血者）血清中的参考值为13 - 79 U/1（±2标准差），平均值为46 U/1。收集的尿液中的值未呈现正态分布；男性的最小值和最大值范围为58 - 385 U/1，女性为7 - 318 U/1。测定了酶促试验的动力学曲线，并研究了葡萄糖的影响和线性关系。与酶促试验相比，对患者的血清和尿液进行了罗氏显色法Amlyochrom检测。血清中的相关系数r = 0.975，尿液中的r = 0.965。
