Zhu Yuan-Yuan, Liang Hong-Wei, Li Zhong, Luo Xiang-Zhong, Li Lin, Zhang Zhi-Wei, Zou Gui-Wei
Fisheries College of Huazhong Agricultural University, Wuhan, China.
Yi Chuan. 2012 Jan;34(1):72-8. doi: 10.3724/sp.j.1005.2012.00072.
Myostatin (MSTN) gene has negative influence on the growth of muscle, which belongs to transforming growth factor-β family. Using single-strand conformation polymorphism (SSCP), single nucleotide polymorphisms (SNPs) of MSTN gene were detected in yellow catfish. Correlation analysis between MSTN polymorphisms and growth traits was also carried out. Results indicated that five genotypes (AA, BB, CC, CD and DD) were found in the first intron with one deletion site and two mutant sites (T1003del, G1022A and T1063G), two genotypes (EE and EF) were found in the third exon with one mutant site (T132C). The individuals with genotypes AA had a lager value than the individuals with the genotype CD and DD in total length, body length, body height, body width, head length and body weight (Plt;0.05). The female individuals with genotypes AA also had a lager value than the female individuals with genotypes DD in total length, body length, body height, body width, caudal peduncle height, caudal peduncle width and body weight (Plt;0.05). This implies that genotype AA has a positive influence on the growth traits of female yellow catfish, and genotype DD is opposite. Genotypes AA and DD can be used in marker assisted selection of female yellow catfish breeding in the future.
肌肉生长抑制素（MSTN）基因对肌肉生长具有负面影响，它属于转化生长因子-β家族。利用单链构象多态性（SSCP）技术，检测了黄颡鱼MSTN基因的单核苷酸多态性（SNP）。同时还进行了MSTN基因多态性与生长性状的相关性分析。结果表明，在第一内含子中发现了5种基因型（AA、BB、CC、CD和DD），存在1个缺失位点和2个突变位点（T1003del、G1022A和T1063G）；在第三外显子中发现了2种基因型（EE和EF），存在1个突变位点（T132C）。基因型为AA的个体在全长、体长、体高、体宽、头长和体重方面的值均大于基因型为CD和DD的个体（P<0.05）。基因型为AA的雌性个体在全长、体长、体高、体宽、尾柄高、尾柄宽和体重方面的值也大于基因型为DD的雌性个体（P<0.05）。这表明基因型AA对雌性黄颡鱼的生长性状有积极影响，而基因型DD则相反。基因型AA和DD未来可用于雌性黄颡鱼育种的分子标记辅助选择。
