Fraile A, García-Arenal F
Departamento de Patología Vegetal, E.T.S.I. Agrónomos, Ciudad Universitaria, Madrid, Spain.
J Gen Virol. 1990 Oct;71 ( Pt 10):2223-8. doi: 10.1099/0022-1317-71-10-2223.
The products of partial proteolysis of the Mr 126,000 in vitro translation products of the RNA of eight tobamoviruses were separated by SDS-polyacrylamide gel electrophoresis. The peptide patterns obtained were compared using a computer program designed to establish phylogenetic relationships. The resulting most-parsimonious phylogenetic trees grouped the tobamoviruses into clusters I (tobacco mosaic virus, tomato mosaic virus, tobacco mild green mosaic virus, pepper mild mottle virus) and II (sunn-hemp mosaic virus, cucumber green mottle mosaic virus, kyuri green mottle mosaic virus), with ribgrass mosaic virus in an intermediate position. This clustering resembles that obtained when the coat proteins of these viruses are compared. If the tobamoviruses have arisen by divergence from an ancestral type, the results suggest that different parts of the genome have diverged similarly and that recombination has not played a major role in the evolution of the group.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对8种烟草花叶病毒RNA的126,000道尔顿体外翻译产物的部分蛋白水解产物进行了分离。使用一个旨在建立系统发育关系的计算机程序对所得的肽图谱进行了比较。由此得到的最简约系统发育树将烟草花叶病毒分为簇I（烟草花叶病毒、番茄花叶病毒、烟草轻绿花叶病毒、辣椒轻斑驳病毒）和簇II（印度麻花叶病毒、黄瓜绿斑驳花叶病毒、瓠瓜绿斑驳花叶病毒），而车前草花叶病毒处于中间位置。这种聚类类似于比较这些病毒的外壳蛋白时所得到的聚类。如果烟草花叶病毒是由一个祖先类型分化而来，那么结果表明基因组的不同部分以类似方式发生了分化，并且重组在该病毒组的进化中未起主要作用。
