Departamento de Biotecnología Agrícola, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional-Unidad Sinaloa del IPN, Guasave, Sinaloa, 81101, Mexico.
J Sci Food Agric. 2012 Jul;92(9):1974-81. doi: 10.1002/jsfa.5570. Epub 2012 Feb 3.
The potential use of hard-to-cook (hardened) chickpeas to obtain value-added functional food ingredients was evaluated. For that purpose, some nutraceutical and functional attributes of several chickpea protein hydrolysates (CPHs) prepared from both fresh and hard-to-cook grains were evaluated.
All the CPHs prepared from both fresh and hard-to-cook grains, with the enzymes alcalase, pancreatin and papain, showed high angiotensin converting enzyme inhibitory (ACE-I) activity with IC₅₀ values ranging from 0.101 to 37.33 µg mL⁻¹; similarly, high levels of antioxidant activity (around 18.17-95.61 µmol Trolox equivalent antioxidant capacity µg⁻¹ CPH) were obtained through both the 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) methods. Regarding functional characterization of the CPHs, oil absorption values ranged from 1.91 to 2.20 mL oil g⁻¹ CPH, with water solubility almost 100% from pH 7 to 10.
The high antioxidant and ACE-I activities as well as the good functional properties of the CPH prepared from both fresh and hard-to-cook grains, suggest its use in food formulations with value added in human health.
评估了难煮（硬化）鹰嘴豆用于获得增值功能性食品成分的潜力。为此，评估了几种由新鲜和难煮谷物制备的鹰嘴豆蛋白水解物（CPH）的一些营养和功能特性。
用碱性蛋白酶、胰蛋白酶和木瓜蛋白酶制备的来自新鲜和难煮谷物的所有 CPH 均表现出高血管紧张素转化酶抑制（ACE-I）活性，IC₅₀ 值范围为 0.101 至 37.33 µg mL⁻¹；同样，通过 2,2-二苯基-1-苦基肼和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）方法获得了高抗氧化活性（约 18.17-95.61 µmol Trolox 当量抗氧化能力 µg⁻¹ CPH）。关于 CPH 的功能特性，吸油值范围为 1.91 至 2.20 mL 油 g⁻¹ CPH，在 pH 7 至 10 时几乎 100%水溶性。
由新鲜和难煮谷物制备的 CPH 具有高抗氧化和 ACE-I 活性以及良好的功能特性，表明其可用于具有附加值的人类健康食品配方中。
