Department of Genetics, Faculty of Biology, Sofia University St. Kliment Ohridski, Sofia, Bulgaria.
Mol Biol Rep. 2012 Jun;39(6):7025-30. doi: 10.1007/s11033-012-1533-z. Epub 2012 Feb 4.
In this study we report a novel method for identification of Enterococcus faecalis based on polymerase chain reaction with primers specific for the eda-genes encoding the enzymes involved in the Entner-Doudoroff pathway, a pathway present only in this species among Gram-positive bacteria. The designed primers were checked in several different Enterococcus species, and with some other Gram-positive and Gram-negative bacterial species as well. Five primer combinations were used to detect the eda-1 gene, and another three for the eda-2 gene. With the exception of one of the primer combinations, all the others gave as results the expected amplification products only in E. faecalis strains.
在这项研究中，我们报告了一种基于聚合酶链反应（PCR）的新型方法，用于鉴定肠球菌属粪肠球菌，该方法使用的引物针对参与 Entner-Doudoroff 途径的酶的 eda 基因，该途径仅存在于革兰氏阳性菌中的这种物种中。设计的引物在几种不同的肠球菌属物种以及其他一些革兰氏阳性和革兰氏阴性细菌物种中进行了检查。使用了五种引物组合来检测 eda-1 基因，另外三种用于检测 eda-2 基因。除了一种引物组合外，其他所有引物组合的结果仅在粪肠球菌菌株中得到预期的扩增产物。
