Angeletti S, Lorino G, Gherardi G, Battistoni F, De Cesaris M, Dicuonzo G
Libera Università Campus Bio-Medico, 00155 Rome, Italy.
J Clin Microbiol. 2001 Feb;39(2):794-7. doi: 10.1128/JCM.39.2.794-797.2001.
For 279 clinically isolated specimens identified by commercial kits as enterococci, genotypic identification was performed by two multiplex PCRs, one with ddl(E. faecalis) and ddl(E. faecium) primers and another with vanC-1 and vanC-2/3 primers, and by 16S ribosomal DNA (rDNA) sequencing. For 253 strains, phenotypic and genotypic results were the same. Multiplex PCR allowed for the identification of 13 discordant results. Six strains were not enterococci and were identified by 16S rDNA sequencing. For 5 discordant and 10 concordant enterococcal strains, 16S rDNA sequencing was needed. Because many supplementary tests are frequently necessary for phenotypic identification, the molecular approach is a good alternative.
对于279份经商业试剂盒鉴定为肠球菌的临床分离标本，通过两种多重聚合酶链反应（PCR）进行基因型鉴定，一种使用ddl（粪肠球菌）和ddl（屎肠球菌）引物，另一种使用vanC-1和vanC-2/3引物，并通过16S核糖体DNA（rDNA）测序进行鉴定。对于253株菌株，表型和基因型结果相同。多重PCR鉴定出13个不一致的结果。6株不是肠球菌，通过16S rDNA测序得以鉴定。对于5株不一致和10株一致的肠球菌菌株，需要进行16S rDNA测序。由于表型鉴定通常需要许多补充试验，分子方法是一个很好的选择。
