Aybeke Mehmet, Sidal Uour
Department of Biology, Faculty of Sciences, Trakya University, Balkan Campus, 22030 Edirne, Turkiye.
Pak J Biol Sci. 2011 Jul 1;14(13):703-8. doi: 10.3923/pjbs.2011.703.708.
The aim of this study is to assess the effects of Olive Oil Mill Wastewater (OOMW) application as irrigation water on in vitro pollen germination, focusing on total protein quantity. In test groups, pollen germination substances such as sucrose, H3BO3 and Ca(NO3)2 were added to different concentrations of OOMW and used as germination media. Regarding control group, the same substance melted into water instead of OOMW. As a result, in general, pollen germination percentage was decreased significantly in all OOMW concentrations than that of the control group, except 1/1000 concentrations. Similarly, total protein quantities declined linearly depending on decreasing OOMW concentrations, except 1/1 concentration which has 4-5 times the control value. Consequently, it was established that OOMW generally decreased pollen germination ratio and had carcinogenic effects on protein synthesis mechanism and must not be used as irrigation water without purification.
本研究的目的是评估将橄榄油厂废水（OOMW）用作灌溉水对体外花粉萌发的影响，重点关注总蛋白量。在试验组中，将蔗糖、H3BO3和Ca(NO3)2等花粉萌发物质添加到不同浓度的OOMW中，并用作萌发培养基。对照组则是将相同物质溶于水而非OOMW。结果表明，总体而言，除1/1000浓度外，所有OOMW浓度下的花粉萌发率均比对照组显著降低。同样，总蛋白量随OOMW浓度降低呈线性下降，但1/1浓度的总蛋白量是对照组的4至5倍。因此，已确定OOMW通常会降低花粉萌发率，并对蛋白质合成机制产生致癌作用，未经净化不得用作灌溉水。
