Swedish Centre for Resource Recovery, University of Borås, Borås, Sweden.
Department of Molecular Biology and Genetics, Gebze Technical University, Gebze-Kocaeli, Turkey.
Bioengineered. 2022 Mar;13(3):7659-7669. doi: 10.1080/21655979.2022.2050492.
In this study, olive oil mill wastewater (OOMW), an important waste in the Mediterranean basin, was evaluated to produce bacterial cellulose (BC). For this purpose, the effects of different ratios of OOMW fractions (25-100%) and some additional nutrients (yeast extract, peptone and Hestrin-Schramm medium (HS) components) on BC productions were investigated. Unsupplemented OOMW medium (75% and 100%) yielded as much as BC obtained in HS medium (0.65 g/L), while enrichment of OOMW medium (%100) with yeast extract (5 g/L) and peptone (5 g/L) increased the amount of BC by 5.5 times, reaching to 5.33 g/L. In addition, produced BCs were characterized by FT-IR, TGA, XRD and SEM analyses. BC from OOMW medium (100% OOMW with supplementation) has a high thermal decomposition temperature (316.8°C), whereas it has lower crystallinity index (57%). According to the FT-IR analysis, it was observed that the components of OOMW might be absorbed by BCs. Thus, higher yield productions of BCs from OOMW media compared to BC obtained from HS medium indicate that olive oil industry wastes can be integrated into BC production for industrial applications.
在这项研究中，评估了橄榄油厂废水（OOMW），这种在地中海盆地产生的重要废物，以生产细菌纤维素（BC）。为此，研究了不同比例的 OOMW 馏分（25-100%）和一些额外营养素（酵母提取物、蛋白胨和 Hestrin-Schramm 培养基（HS）成分）对 BC 生产的影响。未补充 OOMW 培养基（75%和 100%）产生的 BC 与 HS 培养基（0.65g/L）中产生的 BC 一样多，而用酵母提取物（5g/L）和蛋白胨（5g/L）富集 OOMW 培养基（100%）可将 BC 的量增加 5.5 倍，达到 5.33g/L。此外，还通过 FT-IR、TGA、XRD 和 SEM 分析对所生产的 BC 进行了表征。OOMW 培养基中产生的 BC（100%OOMW 加补充物）具有较高的热分解温度（316.8°C），但其结晶度指数（57%）较低。根据 FT-IR 分析，观察到 OOMW 的成分可能被 BC 吸收。因此，与从 HS 培养基中获得的 BC 相比，OOMW 培养基中 BC 的产量更高，这表明橄榄油工业废物可以整合到 BC 生产中，用于工业应用。
